Abstract:Abstract:Monoclonal antibodies against Pasteurella multocida toxin(PMT) were produced by using E.coli expressed recombinant PMT proteins as immunogens.on the basis of recombinant protein expressed by E.coli and HRP-labled monoclonal antibody against Pasteurella multocida toxin,a competitive ELISA was developed to detect antibodies against.Pasteurella multocida toxin.Optimium conditions of the ELISA Were developed as following:the concentration of recombinant for coating ELISA plates is 223 ng /mL;the best dilution of serum to be tested was 1:2; the working titer for HRP-labelled monoclonal antibody was 1:3200;>50% of inhibition rate was the positive judging standard.A total of 82 serum samples were detected in parallel by both the competitive ELISA and in vitro neutralization assay.40.2% of them were detected as positive by competitive ELISA,while 36.6% of them were positive by in vitro neutralization assay.The coincidence rate of the two assays is 91.5%.These results showed that the cELISA has the advantage of higher sensitivity,specificity,reproducibility and stability.It would be very useful in diagnosis,surveillance of PMT antibodies and epidemiological survey.
向敏 卢顺. 检测多杀性巴氏杆菌毒素抗体的单抗竞争ELISA方法的建立[J]. , 2008, 16(4): 0-.
. Establishment of a McAb-based Competitive ELISA for the detection of antibodies against Pasteurella multocida toxin. , 2008, 16(4): 0-.