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2025年8月15日 星期五
  2017, Vol. 25 Issue (11): 1809-1819    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
葡萄LOX基因的克隆及表达分析
刘敏1,鞠延仑1,闵卓1,赵现方1,陈腾1,房玉林2
1. 西北农林科技大学葡萄酒学院
2. 西北农林科技大学
Cloning and Expression Analysis of Lipoxygenase Gene in Grape (Vitis vinifera)
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摘要 绿叶挥发物(green leaf volatiles, GLV)包括C6和C9醛类、醇类和脂类,是酿酒葡萄(Vitis vinifera)和葡萄酒中一类重要的香气成分。脂氧合酶(lipoxygenase, LOX)是合成GLV的关键酶。本研究从酿酒葡萄赤霞珠果实中克隆VvLOX1(KF033130)和VvLOX2(KF033131)基因cDNA全长,经生物信息学分析得出,二者分别属于Ⅰ型9-LOX和Ⅱ型13-LOX。这2个基因在葡萄叶片和根中的表达显著高于卷须和茎中的表达。在葡萄果实发育过程中,VvLOX1和VvLOX2基因表达与GLV合成有关,但基因表达高峰比C6和C9物质含量高峰提前2周出现。创伤处理后VvLOX2基因表达量与C6物质含量呈显著正相关,说明该基因在植物抗逆反应中发挥重要作用。本研究结果为探讨LOX基因在葡萄香气形成中的作用机理提供基础资料。
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刘敏
鞠延仑
闵卓
赵现方
陈腾
房玉林
关键词 葡萄脂氧合酶(LOX)绿叶挥发物(GLV)基因克隆序列分析    
Abstract:Green leaf volatiles (GLV), including C6 and C9 aldehydes, alcohols and lipids, are important aroma components in grapes(Vitis vinifera L). and wines. Lipoxygenase (LOX) is a key enzyme for the synthesis of GLVs. In this study, full-length cDNAs of VvLOX1 (KF033130) and VvLOX2 (KF033131) were cloned from Cabernet Sauvignon(V. vinifera) berries. By bioinformatics analysis, these two genes belonged to type Ⅰ 9-LOX and type Ⅱ 13-LOX, respectively. The expression levels of VvLOX1 and VvLOX2 were higher in leaves and roots than those in stems and tendrils. During the development of grape berries, the expression levels of VvLOX1 and VvLOX2 were related to GLV synthesis, but the peaks of two genes expression appeared 2 weeks earlier than those of C6 and C9 contents. After wounding treatment, there was a significant positive correlation between VvLOX2 expression level and C6 content, which indicated that this gene played an important role in plant stress resistance. The results of this study provide the basic data for exploring the function mechanism of LOXs in aroma formation of grape berries.
Key wordsGrape (Vitis vinifera)    Lipoxygenase(LOX)    Green leaf volatiles(GLV)    Gene cloning    Sequence analysis
收稿日期: 2017-04-10      出版日期: 2017-11-01
基金资助:优质特色酿酒葡萄生产精准水肥调控研究;国家现代农业(葡萄)产业技术体系建设专项资助项目;陕西省重大农技推广服务试点资助项目;杨凌示范区农业科技示范推广基地资助项目
通讯作者: 房玉林     E-mail: fangyulin@nwsuaf.edu.cn
引用本文:   
刘敏 鞠延仑 闵卓 赵现方 陈腾 房玉林. 葡萄LOX基因的克隆及表达分析 [J]. , 2017, 25(11): 1809-1819.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2017/V25/I11/1809
 
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