Occurrence of Apple scar skin disease in Xinjiang and Detection of Apple scar skin viroid by Reverse Transcription - Polymerase Chain Reaction ( RT-PCR )
Abstract：Three apple samples were collected from Xinjiang province with rugose symptoms. The low molecular weight RNAs from three samples were characterized by Return-PAGE. The results showed that two of samples were infected by viroid. Then, it was confirmed to be ASSVd by reverse transcription-polymerase chain reaction (RT-PCR) using two pairs of primers designed according to the reported sequence of ASSVd in GenBank, the amplified products were cloned into pGEM-T and sequenced. Two sequences variants were registered in GenBank DQ362906, DQ362907, The result shows that ASSVd is a quisispecies which contain many very similar but different variants with high sequence homology.
