Abstract：Objective The effects of the characteristics of amniotic fluid on the culture of human amniotic fluid stem cells were discussed in this study. Method Amniotic fluid cells were cultured to examined the effect of different factors on the culture of human amniotic fluid stem cells as measured by the numbers of the attachment cells and the attachment time. Result The attachment time was significantly influenced by the gestational age (p<0.05), and the time was longer when the amniotic fluid was collected at the third trimester of gestation. The attachment time was also affected by the blood pollution level. The volume of the amniotic fluid influenced the numbers of the attachment cells and the attachment time, while the size of the cell pellet had no distinct effect on it. Conclusion The gestational age, the blood pollution level and the volume of the amniotic fluid could influence the numbers of the attachment cells and the attachment time at a certain extent.
王晗 窦忠英 王华岩. 羊水标本性状对人源羊水干细胞原代分离培养的影响[J]. , 2008, 16(5): 0-.
. Effect of the Characteristics of Amniotic Fluid on the Culture of Human Amniotic Fluid Stem Cells. , 2008, 16(5): 0-.
