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2025年8月5日 星期二
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催乳素受体基因多态性及其与济宁青山羊高繁殖力关系的研究
储明星
Polymorphism of Prolactin Receptor Gene and Its Relationship with Prolificacy of Jining Grey Goats
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摘要 设计8对引物,采用PCR-SSCP技术检测催乳素受体(prolactin receptor,PRLR)基因外显子3至外显子9和内含子9在高繁殖力山羊品种(济宁青山羊)和低繁殖力山羊品种(辽宁绒山羊、安哥拉山羊)中的单核苷酸多态性,同时研究该基因对济宁青山羊高繁殖力的影响。结果表明:8对引物中,只有扩增内含子9的P8引物的扩增区域具有多态性,其余7对引物的扩增片段都不存在多态性。对于P8扩增片段,在济宁青山羊、辽宁绒山羊和安哥拉山羊中都只检测到AA型和AB型。 测序表明AB型与AA型相比有1处突变(209A→G),该突变位于PRLR的第9内含子。济宁青山羊2 种基因型之间产羔数的最小二乘均值差异不显著(P>0.05)。这些结果初步表明检测的PRLR基因位点对济宁青山羊高繁殖力没有显著影响。
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储明星
关键词 山羊高繁殖力催乳素受体基因    
Abstract:Prolactin receptor(PRLR)gene was studied as a candidate gene for the prolificacy of Jining Grey goats. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 3 to exon 9 and intron 9 of PRLR gene in both high fecundity breed (Jining Grey goat) and low fecundity breeds (Liaoning Cashmere goat and Angora goat) by PCR-SSCP. Only the products amplified by primer P8 amplifying the intron 9 displayed polymorphisms. For primer P8, only two genotypes (AA and AB) were detected in three goat breeds. Sequencing revealed one mutation (209A→G) of PRLR gene in the genotype AB in comparison to the genotype AA, and this mutation was situated in intron 9 of PRLR gene. The difference of the least squares means for litter size between AA and AB was non-significant (P>0.05) in Jining Grey goats. These results preliminarily showed that these detected loci of PRLR gene had no significant effect on prolificacy of Jining Grey goats.
Key wordsgoat    prolificacy    prolactin receptor gene
收稿日期: 2007-04-03     
通讯作者: 储明星   
引用本文:   
储明星. 催乳素受体基因多态性及其与济宁青山羊高繁殖力关系的研究[J]. , 2008, 16(4): 0-.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2008/V16/I4/0
 
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