Development of Flax (Linum usitatissimum) InDel Markers Based on Resequencing and Analysis of Genetic Diversity and Population Structure in Its Cultivars
WANG Bin1, LIU Jie2, HOU Jing-Jing1, ZHAO Li1,*
1 Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：InDel markers have been widely used in research fields such as genetic diversity analysis of crop germplasm resources and molecular marker-assisted breeding, but they are scarcely reported in flax (Linum usitatissimum) related studies. This study used resequencing data of 2 flax materials to identify InDel loci, develop polymorphic InDel markers and genotyped 93 flax cultivars to investigate the genetic diversity, phylogenetic relationships, and population genetic structure characteristics of flax cultivars. The results showed that, identified 66 851 InDel loci and designed 120 InDel markers based on the whole genome re-sequencing data, and 26 pairs of primers with good polymorphism were screened to analyze genetic diversity and population structure of 93 flax varieties. A total of 80 alleles were detected with an average of 3.08 alleles per locus, the gene diversity index and polymorphism information content ranged from 0.169 6 to 0.694 2 and 0.161 5 to 0.636 9, respectively, with an average of 0.485 2 and 0.415 0 respectively. All varieties were divided into 2 groups with cluster analysis, the fiber flax were clustered into one groups, the oil flax were divided into one groups; population structure analysis divided all varieties into 4 groups; principal component analysis separated fiber flax from oil flax; The results of analysis of molecular variance (AMOVA) showed that the variation mainly occurred within individuals and among individuals, there was also a certain amount of variation among populations. The results of cluster analysis and principal component analysis were consistent, indicated that genetic diversity of fiber flax populations was higher than that of oil flax populations, the genetic variation of oil flax was independent of geographical region. The results can reveal the relationship of 93 cultivated flax varieties, which has certain theoretical significance and application value of identification flax varieties, analysis flax resources' relationship and molecularly assisted breeding.
王斌, 刘杰, 侯静静, 赵利. 基于重测序的亚麻InDel标记开发及亚麻育成品种遗传多样性和群体结构分析[J]. 农业生物技术学报, 2026, 34(1): 78-87.
WANG Bin, LIU Jie, HOU Jing-Jing, ZHAO Li. Development of Flax (Linum usitatissimum) InDel Markers Based on Resequencing and Analysis of Genetic Diversity and Population Structure in Its Cultivars. 农业生物技术学报, 2026, 34(1): 78-87.
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