Abstract：DNAJ protein usually plays an important role in plant growth and development and resistance to biological and non-biological stress as an auxiliary companion molecule of heat shock protein70 (HSP70). A total of 10 melon (Cucumis melo) DNAJ candidate members were identified in the transcriptome sequencing data of melon seedlings after low-temperature treatment by bioinformatics methods in this study.Furthermore, chromosome position, gene structure, subcellular localization, protein secondary/tertiary structure were determined. Meanwhile, the protein interaction, systematic evolution, cis-acting element and transcription level under abiotic stresses were analyzed. Results showed that 4 CmDNAJ members were distributed on 3 chromosomes, and were named CmDNAJ1~CmDNAJ4. The length of amino acids ranged from161 to 503 aa, all of which were hydrophilic, and located in the nucleus (CmDNAJ2/3), cytoplasmic (CmDNAJ12/3/4) and endoplasmic reticulum (CmDNAJ2). Systematic evolutionary analysis found that members of the melon DNAJ gene family were closely related to cucumber (C. sativus). The predictiveanalysis found that the 4 DNAJ promoters mainly contained cis-acting elements such as hormone response, light response and abiotic stress response. There were significant differences in tissue-specific expression of the 4 genes, only CmDNAJ1 and CmDNAJ2 showing highly expressed in male flowers, female flowers, roots,fruits and leaves of melons. CmDNAJ1, CmDNAJ2 and CmDNAJ3 might play a positive regulatory role in the process of fruit development and maturity, while CmDNAJ4 might play a negative regulatory role. The results of qRT-PCR confirmed that CmDNAJ1~CmDNAJ4 all responded to low temperature, high salt, abscisic acid (ABA), and drought induction. CmDNAJ1 and CmDNAJ2 were the key gene for responding to cold stress; CmDNAJ2 and CmDNAJ3 were the key genes for responding to salt stress, ABA stress and drought stress. Thisstudy provides a theoretical reference for analyzing the DNAJ gene function of melon.
汪涛, 嵇泽琳, 杜清洁, 李娟起, 汪虎, 王吉庆, 肖怀娟, 李猛. 基于转录组测序的甜瓜DNAJ基因家族鉴定与表达分析[J]. 农业生物技术学报, 2025, 33(6): 1234-1245.
WANG Tao, I Ze-Lin, DU Qing-Jie, LI Juan-Qi, WANG Hu, WANG Ji-Qing, XIAO Huai-Juan, LI Meng. Identification and Expression Analysis of DNAJ Gene Family in Melon(Cucumis melo) Based on Transcriptome Sequencing. 农业生物技术学报, 2025, 33(6): 1234-1245.
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