Research Progress on Improvement in Development of Porcine Somatic Cell Nuclear Transfer Embryos by Histone Deacetylase Inhibitors
YAN Chao1,2, CHEN Zhi-Long2, PENG Cui-Ting2, XIE Hao2, ZHANG Cai-Yong2, ZHAO Yu-Lan2, QI Lin2, LIU Yong-Gang1,*, TANG Zhong-Lin2,3,4,5,*
1 College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China; 3 Kunpeng Institute of Modern Agriculture at Foshan, Foshan 528226, China; 4 Shenzhen Branch Center of Guangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Shenzhen 518000, China; 5 Key Laboratory of Livestock and Poultry Biohistology, Ministry of Agriculture and Rural Development, Shenzhen 518000, China
Abstract：Somatic cell nuclear transfer (SCNT) has been widely used in protecting endangered species, propagating better varieties and treating diseases. Histone acetylase is an important epigenetic mark regulating the development of early embryo in pig (Sus scrofa), and its correct obliteration and reconstruction are the basis of the embryonic development. With the development of low input next generation sequencing, many studies found that a variety of abnormal epigenetic modification have been found during the reprogramming of somatic cell nuclear transfer embryos. Histone acetylase modification is an important factor that leads to the development block of porcine somatic cell nuclear transfer embryos. In recent years, some small molecule compounds of histone deacetylase inhibitors have been gradually discovered for the improvement of abnormal modification level of histone acetylase during embryonic development. This paper reviews the studies of the effects and mechanisms of histone deacetylase inhibitors on the development of porcine somatic cell nuclear transfer embryos, which provides reference for improving the cloning efficiency in pig.
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