Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education/Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction/College of Zoology, Guizhou University, Guiyang 550025, China
Abstract：RhoGTPase activating protein 24 (ARHGAP24) gene is closely related to actin remodeling, cell polarity control and cell migration, and this study aims to explore the relationship between ARHGAP24 gene polymorphisms and the growth traits of Xiangsu hybrid pigs Sus scrofa, so as to provide a reference for the molecular marker selection of Xiangsu hybrid pigs. In this study, the blood DNA of 164 Xiangsu hybrid pigs under the same feeding conditions was collected, and the SNP sites of ARHGAP24 genes were determined by PCR amplification and sequencing. The general linear model in SPSS 25.0 software was used to analyze the association between the SNP site of the ARHGAP24 gene and the growth traits of Xiangsu hybrid pigs. A total of 5 SNP sites were detected in 9 exons of Xiangsu hybrid pigs, located in exon 1 and exon 8, respectively.g.735024 A>G nonsynonymous mutations caused valine at position 455 to change to aspartic acid on mRNA, which had no obvious effect on the structure of mRNA, but leaded to the α-helix and irregular coil of the protein secondary structure, and the decrease of β-corner and extension chain. After the nonsynonymous mutation of the g.735242 A>C site, the 528th position valine changed to serine, which produced obvious changes in the mRNA structure, while the proportion of α-helix and extended chain in the secondary structure of the protein decreased slightly, and the proportion of β-turn angle and irregular curl increased slightly. The analysis of association results showed that the C>T locus of g.735313 had significant effect on chest circumference (P<0.01) and abdominal circumference (P<0.05). Linkage imbalance analysis of 5 SNPs sites detected 5 haplotypes and 15 doubles. The g.735313 C>T loci in the ARHGAP24 gene of Xiangsu hybrid pigs were significantly associated with the chest and abdominal circumference of Xiangsu hybrid pigs, and were moderately polymorphic and in Hardy-Weinberg equilibrium, which could be used as candidate sites for molecular breeding of genetic markers of Xiangsu hybrid pigs in the later stage.
[1] 李隐侠, 牙生江·那斯尔, 赛里克·都曼, 等. 2023. SNP芯片评估柯尔克孜羊群体遗传多样性和遗传结构[J]. 畜牧兽医学报, 54(02): 572-583. (Li Y X, Ya S J, Sai L D, et al.2023. Evaluation of genetic diversity and genetic structure of Kirgiz sheep population based on SNP chip[J]. Acta Veterinaria et Zootechnica Sinica, 54(02): 572-583.) [2] 毛圆辉. 2015. 翻译过程中mRNA二级结构的功能研究[D]. 博士学位论文, 西北农林科技大学, 导师: 陶士珩, pp. 1. (Mao Y H.2015. A systematic analysis of mRNA secondary structure during translation[D]. Thesis for Ph.D., Northwest A&F University, Supervisor: Tao S H, pp. 1.) [3] 倪萌萌, 谢玲玲, 阮涌, 等. 2018. 从江香猪与苏太猪不同组合杂交性能比较研究[J]. 畜牧与兽医, 50(10): 12-16. (Ni M M,Xie L L, Ruan Y, et al.2018. Comparative study of the crossing performance of different combinations of Congjiang Xiang pigs and Sutai pigs[J].Animal Husbandry & Veterinary Medicine, 50(10): 12-16.) [4] 覃媛钰, 张依裕, 罗华伦, 等. 2019. SFRP5基因遗传变异对长顺绿壳蛋鸡胸肌肌肉品质的影响[J]. 农业生物技术学报, 27(04): 703-711. (Qin Y Y, Zhang Y Y, Luo H L, et al.2019. Effects of genetic variation of SFRP5 gene on breast muscle quality of Changshun blue-eggshll chicken[J].Journal of Agricultura Biotechnology, 27(04): 703-711.) [5] 吴和燕, 高春林, 卢枚, 等. 2018. 局灶节段性肾小球硬化的流行病学进展[J]. 临床儿科杂志, 36(09): 716-719. (Wu H Y, Gao C L, Lu M, et al.2018. Progress in epidemiology of focal segmental glomerulosclerosis[J]. The Journal of Clinical Pediatrics, 36(09): 716-719.) [6] 吴培, 赵宗胜, 何开兵, 等. 2023. 奶牛隐性乳房炎抗性相关基因多态性与体细胞评分关联分析[J]. 黑龙江畜牧兽医, (01): 68-77. (Wu P, Zhao Z S, He K B, et al. 2023. Association analysis of recessive mastitis resistance-related gene polymorphisms with somatic cell scores in dairy cows[J]. Heilongjiang Animal Science and Veterinary Medicine, (01): 68-77.) [7] 许家利, 许厚强, 阮涌, 等. 2022. F3代香苏杂交猪DKK1基因多态性与生长性状的关联性[J]. 南方农业学报, 53(04): 969-976. (Xu J L, Xu H Q, Ruan Y, et al.2022. Correlation analysis of DKK1 gene polymorphism andgrowth traits of F3 generation Xiangsu hybrid pigs[J].Journal of Southern Agriculture, 53(04): 969-976.) [8] 薛倩, 王金玉, 张跟喜, 等. 2015. 黑素皮质素受体3基因(MC3R)多态性及其单倍型组合与京海黄鸡屠体性状的关联分析[J]. 农业生物技术学报, 23(03): 344-351. (Xue Q, Wang J Y, Zhang G X, et al.2015. Polymorphism of melanocortin 3 receptor gene (MC3R) and association analysis between the diplotypes and the carcass traits in jinghai yellow chicken[J]. Journal of Agricultural Biotechnology, 23(03): 344-351.) [9] 杨伟杰, 满初日嘎, 吴慧, 等. 2022. 海南黄牛IGF2R基因单核苷酸多态性及其生物信息学分析[J]. 南方农业学报, 53(12): 3520-3528. (Yang W J, Manchu R G, Wu H, et al.2022. Single nucleotide polymorphism and bioinformatics analysis of IGF2R gene in Hainan cattle[J].Journal of Southern Agriculture, 53(12): 3520-3528.) [10] Akilesh S, Suleiman H, Yu H, et al.2011. Arhgap24 inactivates Rac1 in mouse podocytes, and a mutant form is associated with familial focal segmental glomerulosclerosis[J]. The Journal of Clinical Investigation, 121(10): 4127-4137. [11] Azimu W, Manatbay B, Li Y, et al.2018. Genetic diversity and population structure analysis of eight local chicken breeds of Southern Xinjiang[J]. British Poultry Science, 59(6): 629-635. [12] Feng M, Bao Y, Li Z, et al.2014. RASAL2 activates RAC1 to promote triple-negative breast cancer progression[J]. The Journal of Clinical Investigation, 124(12): 5291-5304. [13] Jin X, Zhang B, Zhang H, et al.2022. Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma[J]. Oncogene, 41(4): 538-549. [14] Katoh Masuko, Katoh Masaru, 2004. Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico[J]. International Journal of Molecular Medicine, 14(2): 333-338. [15] Liu H, Wang W, Shen W, et al.2020. ARHGAP24 ameliorates inflammatory response through inactivating Rac1/Akt/NF-kappaB pathway in acute pneumonia model of rat[J]. Annals of Translational Medicine, 8(20): 1289. [16] Maki-Tanila A, Webster L.2019. Heritability, SNP, inbreeding, dairy cattle, genomic selection-and other keywords[J]. Journal of Animal Breeding and Genetics, 136(1): 1-2. [17] Nakamura F.2013. FilGAP and its close relatives: A mediator of Rho-Rac antagonism that regulates cell morphology and migration[J]. Biochemical Journal, 453(1): 17-25. [18] Ohta Y, Hartwig J H, Stossel T P.2006. FilGAP, a Rho- and ROCK-regulated GAP for Rac binds filamin A to control actin remodelling[J]. Nature Cell Biology, 8(8): 803-814. [19] Santos C P, Aguiar A F, Giometti I C, et al.2018. High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats[J]. Lasers in Medical Science, 33(4): 843-850. [20] Wang L, Shen S, Wang M, et al.2019. Rho GTPase activating protein 24 (ARHGAP24) silencing promotes lung cancer cell migration and invasion by activating beta-catenin signaling[J]. Medical Science Monitor, 25: 21-31. [21] Wang L, Shen S, Xiao H, et al.2020a. ARHGAP24 inhibits cell proliferation and cell cycle progression and induces apoptosis of lung cancer via a STAT6-WWP2-p27 axis[J]. Carcinogenesis, 41(5): 711-721. [22] Wang L, Yang X, Wan L, et al.2020b. ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42[J]. Clinical and Experimental Pharmacology and Physiology, 47(9): 1591-1599. [23] Xu G, Lu X, Huang T, et al.2016. ARHGAP24 inhibits cell cycle progression, induces apoptosis and suppresses invasion in renal cell carcinoma[J]. Oncotarget, 7(32): 51829-51839. [24] Xu Q, Zhao J, Guo Y, et al.2022. A single-nucleotide polymorphism in the promoter of porcine ARHGAP24 gene regulates aggressive behavior of weaned pigs after mixing by affecting the binding of transcription factor p53[J]. Frontiers in Cell and Developmental Biology, 10: 839583. [25] Yang W, Wang B, Yu Q, et al.2022. ARHGAP24 represses beta-catenin transactivation-induced invasiveness in hepatocellular carcinoma mainly by acting as a GTPase-independent scaffold[J]. Theranostics, 12(14): 6189-6206.
