联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月6日 星期日
农业生物技术学报  2024, Vol. 32 Issue (2): 273-282    DOI: 10.3969/j.issn.1674-7968.2024.02.003
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
磷转运蛋白StPHO1.3对马铃薯耐热性的影响及其互作蛋白的筛选
李万1,*, 李成2, 程敏3, 吴芳4
1 商洛学院 生物医药与食品工程学院,商洛 726000;
2 西安润壮稼农业科技有限公司,西安 710003;
3 商洛学院 科技处,商洛 726000;
4 商洛学院 国内合作与校友工作处,商洛 726000
Effects of Phosphorus Transporter StPHO1.3 on Heat Resistance of Potato (Solanum tuberosum) and Screening of Its Interacting Proteins
LI Wan1,*, LI Cheng2, CHENG Min3, WU Fang4
1 School of Biomedicine and Food Engineering, Shangluo University, Shangluo 726000, China;
2 Xi'an Runzhuangjia Agricultural Technology Co., Ltd., Xi'an 710003, China;
3 Science and Technology Division, Shangluo University, Shangluo 726000, China;
4 Domestic Cooperation and the Schoolfellow Bureau, Shangluo University, Shangluo 726000, China
全文: PDF (9081 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 植物通过磷转运蛋白(phosphorus transporters, PHO)吸收和转运磷元素,磷元素作为植物生长发育必不可少的营养素之一,对作物产量、品质和抗逆性均有重要影响。高温是影响马铃薯(Solanum tuberosum)产量和品质的重要环境因子,为探究磷转运蛋白对马铃薯耐热性的影响,本研究通过农杆菌(Agrobacterium tumefaciens)介导法获得了过表达磷转运蛋白基因StPHO1.3的马铃薯转基因株系,比较转基因株系与野生型株系在高温(35 ℃)环境下的生长发育情况发现,过表达StPHO1.3能够增强马铃薯耐热性,对马铃薯生长发育具有促进作用。亚细胞定位结果显示,StPHO1.3在细胞膜上表达;通过酵母双杂交在膜系统文库质粒中筛选StPHO1.3的互作蛋白,并通过双分子荧光互补(bimolecular fluorescence complementation, BiFC)技术进一步验证,结果表明,StPHO1.3与光系统Ⅱ蛋白亚基和钙离子转运相关蛋白均有相互作用。综上所述,过表达StPHO1.3可能通过影响光合作用和信号转导,从而增强马铃薯抵御高温胁迫的能力,促进马铃薯生长发育。本研究为深入理解磷转运蛋白的功能提供了理论依据和参考。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
李万
李成
程敏
吴芳
关键词 马铃薯磷转运蛋白(PHO)高温胁迫酵母双杂交双分子荧光互补(BiFC)    
Abstract:Plants absorb and transport phosphorus through phosphorus transporters (PHO), and phosphorus is one of the essential nutrients for plant growth and development, which has an important effect on crop yield, quality and stress resistance. High temperature is an important environmental factor affecting potato yield and quality. In order to explore the influence of phosphorus transporter on potato (Solanum tuberosum) heat resistance, this study constructed a transgenic potato strain with overexpression of phosphorus transporter gene StPHO1.3 mediated by Agrobacterium tumefaciens. By comparing the growth and development of transgenic strain and wild-type strain at high temperature (35 ℃), it was found that overexpressing StPHO1.3 could enhance the heat resistance and promote the growth and development of potato. In addition, the results of subcellular localization showed that StPHO1.3 was expressed on the cell membrane. Therefore, in this study, the interacting proteins of StPHO1.3 were screened in the membrane system library plasmid by yeast two-hybridization and further verified by bimolecular fluorescence complementation (BiFC). The results showed that, StPHO1.3 interacted with both the photosystem Ⅱ protein subunit and calcium transport-associated proteins. In conclusion, overexpression of StPHO1.3 could enhance the resistance to high temperature stress and promote the growth and development of potato by affecting photosynthesis and signal transduction. These results provide theoretical basis and reference for further understanding of the function of phosphorus transporters and promote the breeding of new potato varieties.
Key wordsSolanum tuberosum    Phosphorus transporter (PHO)    High temperature stress    Yeast two-hybrid    Bimolecular fluorescence complementation (BiFC)
收稿日期: 2023-03-27     
ZTFLH:  S532  
基金资助:陕西省自然科学基础研究计划(2022JQ-223); 陕西省高校科协青年人才托举计划(20220213); 商洛学院博士启动项目(21SKY112); 陕西省科技创新团队(2022TD-56)
通讯作者: *599092122@qq.com   
引用本文:   
李万, 李成, 程敏, 吴芳. 磷转运蛋白StPHO1.3对马铃薯耐热性的影响及其互作蛋白的筛选[J]. 农业生物技术学报, 2024, 32(2): 273-282.
LI Wan, LI Cheng, CHENG Min, WU Fang. Effects of Phosphorus Transporter StPHO1.3 on Heat Resistance of Potato (Solanum tuberosum) and Screening of Its Interacting Proteins. 农业生物技术学报, 2024, 32(2): 273-282.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.02.003     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I2/273
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn