Bioinformatics and Expression Analysis of StbZIP10 Gene Under Zinc Stress in Potato (Solanum tuberosum)
LIU Hao-Tian1, ZHAO Jing2, TANG Xun1, ZHAO Gui-Bin3, ZHU Yong-Yong3, ZHANG Ning1, SI Huai-Jun1,*
1 College of Life Science and Technology/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; 2 Gansu Agricultural Information Center, Lanzhou 730030, China; 3 Gansu General Station of Agro-technology Extension, Lanzhou 730020, China
Abstract：Zinc plays a role in many aspects of plant growth and development. However, excessive zinc and zinc deficiency can lead to physiological and biochemical disorders, forming zinc stress. Basic leucine zipper (bZIP) transcription factors play an important role in regulating plant growth and development and responding to stress. In this study, the full length of the potato (Solanum tuberosum) StbZIP10 gene (GenBank No. XM_006359636.1) was 1 653 bp with an CDS of 819 bp, encoding 272 amino acids. Bioinformatics analysis showed that StbZIP10 was a hydrophobic transmembrane protein with a relative molecular mass of 29.68 kD and had a typical bZIP conserved domain. The subcellular localization results showed that StbZIP10 protein was mainly expressed in the nucleus and cell membrane. The tissue-specific expression analysis of potato StbZIP10 gene was carried out by qRT-PCR. The results showed that the expression level of StbZIP10 gene was the highest in potato roots and the lowest in leaves, and there were significant difference among different plant tissues (P<0.05). The expression pattern of StbZIP10 in different plant tissues under zinc deficiency was the same, and the expression level in roots was the highest between 'Atlantic' and 'Longshu 7'. Under zinc deficiency treatment, the expression of StbZIP10 in both varieties was increased, but the expression of 'Longshu 7 ' was significantly higher than that of 'Atlantic' (P<0.05), and the trend was basically the same in the 3 time periods. The results could provide a theoretical basis for further elucidating the function of the potato StbZIP10 gene.
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