Functional Prediction of circ_MBNL1 in Duck (Anas platyrhynchos domesticus) Adipocyte Differentiation
ZHANG Ao1, WANG Lai-Di1, YANG Ting1, JIANG Yong1, WANG Zhi-Xiu1, CHEN Guo-Hong1,2, BAI Hao2,*, CHANG Guo-Bin1,2,*
1 Key Laboratory of Animal Genetic Breeding and Molecular Design in Jiangsu Province/College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2 Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education/Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China
Abstract：Circular RNAs (circRNAs), a novel endogenous non-coding RNA expressed in various tissues. In this paper, circ_MBNL1 encoded by MBNL1 (musblind-like 1) gene was selected for identification and functional analyses based on the results of circRNA sequencing during the differentiation of duck (Anas platyrhynchos domesticus) embryo primary cells. The conjugated sequence was obtained by PCR amplification and pyrosequencing. And the nucleoplasmic separation assay revealed that duck circ_MBNL1 was mainly localized in the nucleus. Tissue expression profiles suggested that circ_MBNL1 was mainly expressed in duck spleen, adipose and lung tissues. The bioinformatics analysis results indicated that circ_MBNL1 and the predicted target genes were mainly enriched in adipocytokine signaling pathway, FoxO signaling and other pathways, which was hypothesized that circ_MBNL1 might be involved in regulating the differentiation process of duck adipocytes. This study provides a theoretical basis for an in-depth investigation of the regulatory role of circ_MBNL1 in the differentiation process of duck adipocytes.
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