Expression, Localization and Functional Prediction of MVK and PMVK in Mammary Gland of Dairy Cows (Bos taurus) with Clinical Mastitis
ZHANG Bo-Hao1,3, ZHANG Quan-Wei2,3, LIN Ting2,3, DAI Li-Jun2,3, BAI Xu2,3, ZHAO Xing-Xu1,2,3*, ZHANG Yong1,2,3*
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China; 3 Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China
Abstract：Mevalonate kinase (MVK) and phosphovalonate kinase (PMVK), the key enzymes of mevalonate pathway, are considered to be closely related to inflammatory response. However, the role and mechanism of MVK and PMVK in cows (Bos taurus) with clinical mastitis (CM) is unknown. The aim of this study was to explore the expression and distribution of MVK and PMVK, and further investigate their potential function in cows with CM. The mammary gland tissues of Holstein cows in Control (C, n=3) and CM group (n=3) were collected. The expression and distribution of MVK and PMVK in cow mammary gland tissues were analyzed by hematoxylin-eosin (HE) staining, immunohistochemical (IHC) staining, immunofluorescence (IF) staining and qRT-PCR; Meanwhile, based on the data-independent acquisition (DIA) proteomic data, the role of MVK and PMVK in CM was analyzed and discussed through bioinformatics. The results showed that MVK and PMVK were mainly localized in the cytoplasm of epithelial cells; Compared with the C group, the MVK and PMVK gene and its protein levels of in CM group were extremely significantly down regulated (P<0.01); The bioinformatics analysis showed that MVK and PMVK were involved in inflammatory regulation by affecting coenzyme metabolism, amino acid and fatty acid synthesis. In conclusion, the results indicated that MVK and PMVK were correlated with the occurrence and development of CM in cows, which provides important insights into the function and regulatory mechanism of MVK and PMVK in CM of cows.
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