Abstract：Fruit ripening is regulated by a variety of plant hormones, such as ethylene, abscisic acid (ABA) and gibberellin (GA), and ripening-related genes. SlGA2ox (SlGA2-oxidase) family are key genes for regulating GA metabolism and play significant role in regulating tomato (Solanum lycopersicum) fruit ripening. Nevertheless, the specific role of SlGA2ox in maturation-related GA metabolism and its interaction with ethylene and ABA remain unclear. In this study, 15 SlGA2ox genes were screened based on the tomato genome database, and the role of SlGA2ox during maturation process was explored through promoter element prediction, gene expression analysis, and the response analysis to GA, ethylene and ABA. The results showed that 12 SlGA2ox genes were closely related to tomato fruit ripening. Among them, SlGA2ox2, SlGA2ox5, SlGA2ox6 and SlGA2ox8 might be regulated by ethylene in the process of mediating GA metabolism, while SlGA2ox4 and SlGA2ox5 might be regulated by ABA at the same time. In addition, SlGA2ox5 was regulated by both ABA and ethylene signals, so it might be a key gene in the fruit ripening which was regulated jointly by the 3 hormones. The present study provides basic material for in-depth exploration of the genes related to tomato fruit ripening regulation, and provides important clues for exploring the molecular mechanism of tomato fruit ripening regulation.
陈奚潼, 杨军, 何卓远, 曾文静, 周家燕, 王倩, 邹建. 番茄果实成熟过程中GA代谢相关SlGA2ox基因家族受乙烯和ABA调控[J]. 农业生物技术学报, 2023, 31(1): 25-35.
CHEN Xi-Tong, YANG Jun, HE Zhuo-Yuan, ZENG Wen-Jing, ZHOU Jia-Yan, WANG Qian, ZOU Jian. GA Metabolism-related Gene Family SlGA2ox is Regulated by Ethylene and ABA During Tomato (Solanum lycopersicum) Fruit Ripening. 农业生物技术学报, 2023, 31(1): 25-35.
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