宁海黄鸡和广西黄鸡KLF7基因多态性及其与屠体性状的相关分析

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摘要摘要 Kruppel样因子7(Kruppel-like factor, KLF7)是参与脂肪代谢的一个因子，其对鸡(Gallus gallus) 生长和屠体性状的相关研究较少。本研究采用600K Affymetrix Axiom Chicken SNP芯片技术，研究鸡KLF7基因单核苷酸多态性(SNPs)及其与屠体性状的相关性。本实验经对宁海黄鸡和广西黄鸡各59个个体进行基因分型，在KLF7基因上共发现38个SNPs位点，其中4个SNPs位点位于外显子上。除宁海黄鸡T16936C、A18000C、C33569T、T34758C和T40474G位点以及广西黄鸡A18715C和T45125A位点外，其余位点均处于Hardy-Weinberg 平衡状态，多态信息含量显示，宁海黄鸡和广西黄鸡KLF7基因大多数位点属中度多态。各SNPs位点与宁海黄鸡和广西黄鸡屠体性状相关性分析结果表明，分别有7个和6个SNPs位点对部分屠体性状有显著或极显著影响(P＜0.05 或 P＜0.01)。其中，A18000C和T45125A两个SNPs位点对宁海黄鸡和广西黄鸡屠体性状均有显著或极显著影响(P＜0.05 或P＜0.01)且宁海黄鸡有AA、AB和BB 3种基因型，而广西黄鸡仅有AB和BB两种基因型。宁海黄鸡A18000C位点AB型的胸肌率极显著高于BB型(P＜0.01)，T45125A位点AB型的半净膛率和全净膛率极显著高于AA型(P＜0.01)，显著高于BB型(P＜0.05)，AB型的腹脂重和腹脂率显著高于AA型和BB型(P＜0.05)；广西黄鸡A18000C位点AB型的胸肌率显著高于BB型(P＜0.05)，T45125A位点AB型的屠宰率、半净膛率、全净膛重和全净膛率显著高于BB型(P＜0.05)。研究结果表明，KLF7基因对鸡屠体性状有一定的基因效应，为鸡屠体性状具有潜力的候选基因，可作为鸡屠体性状的分子标记辅助选择。

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	董定娟
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关键词 ：单核苷酸多态性, Kruppel样因子7基因(KLF7), 屠体性状, 基因型, 鸡

Abstract：Abstract Kruppel-like factor 7 (KLF7) is a factor involved in fat metabolism and its related research on growth and carcass traits in chicken (Gallus gallus) is less. In order to analyze the association between the single nucleotide polymorphism (SNPs) of KLF7 gene and carcass traits in chicken, the SNPs of the whole genome were genotyped through 600K Affymetrix Axiom Chicken SNP chip technology in Ninghai and Guangxi yellow chickens with each of 59 individuals. Within the total 38 identified SNPs, only 4 SNPs were found in exon regions of KLF7 gene. Most of the detected SNPs revealed 3 genotypes (i.e. AA, AB and BB). The χ2 test showed that the genotypic frequencies of 5 SNPs (i.e. T16936C, A18000C, C33569T, T34758C and T40474G) within Ninghai yellow chicken and 2 SNPs (i.e. A18715C and T45125A) within Guangxi yellow chicken were not in agreement with the Hardy-Weinberg equilibrium (P＜0.05), while the genotype frequencies of the other SNPs in these two chicken populations were all under the Hardy-Weinberg equilibrium (P＞0.05), which showed that there was dynamic equilibrium in two studied chicken populations. According to the classification of polymorphism information content (PIC), the polymorphism at most of the detected SNPs in the current study presented moderately in these 2 chicken breeds. Association studies between each SNP genotype and carcass traits were performed by using the GLM. The results showed that 7 SNPs (i.e. A14371G, A18000C, C28254A, C33569T, T45125A, A56295G and G59074A) of KLF7 gene in Ninghai yellow chicken and 6 SNPs (i.e. C12493G, A18000C, G30642A, A38815G, T45125A and C58906T) in Guangxi yellow chicken were significantly associated with carcass traits (P＜0.05 or P＜0.01). The 2 chicken populations possessed 2 common SNPs (i.e. A18000C and T45125A) which significantly associated with carcass traits (P＜0.05 or P＜0.01). The 2 SNPs (i.e. A18000C and T45125A) revealed 3 genotypes (i.e. AA, AB and BB) in Ninghai yellow chicken, while 2 genotypes (i.e. AB and BB) in Guangxi yellow chicken . Genotype AB of the site A18000C was significantly superior to BB in breast muscle rate in Ninghai yellow chickens (P＜0.01), while the AB genotype of T45125A was significantly superior to AA and BB in semi-eviscerated rate, eviscerated rate, abdominal fat weight and abdominal fat rate (P＜0.05 or P＜0.01). Genotype AB of the site A18000C was significantly superior to BB in breast muscle rate in Guangxi yellow chickens (P＜0.05), while the AB genotype of T45125A was significantly superior to BB in slaughter rate, semi-eviscerated rate, eviscerated weight and eviscerated rate (P＜0.05). The results suggested that KLF7 gene may be one of the candidate genes that control carcass traits, and 2 SNPs (i.e. A18000C and T45125A) could be potential candidate genetic markers for Marker-aid selection in chickens.

Key words： Single nucleotide polymorphisms Kruppel-like factor gene (KLF7) Carcass traits Genotype Chicken

收稿日期: 2016-10-04 出版日期: 2017-01-13

基金资助:浙江省重大科技专项重大农业项目

通讯作者: 尹兆正 E-mail: yzhzh@zju.edu.cn

引用本文:

董定娟曹海月董信阳尹兆正. 宁海黄鸡和广西黄鸡KLF7基因多态性及其与屠体性状的相关分析[J]. , 2017, 25(2): 282-290.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2017/V25/I2/282

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