联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月6日 星期日
  2018, Vol. 26 Issue (12): 2121-2131    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
嗜虫耶尔森氏菌Ye1-8的分离鉴定及其对红棕象甲的杀虫活性
郭雅洁1,韩红2,廖启炓3,林倩男1,牟亚妮4,翁小倩1,邹双全1,张飞萍5,吴松青1
1. 福建农林大学
2.
3. 厦门市绿化管理中心
4. 福建农林大学闽台作物有害生物生态防控国家重点实验室
5. 福建农林大学林学院
Isolation and Identification of Yersinia entomophaga Strain Ye1-8 and Its Toxicity to the Red Palm Weevil (Rhynchophorus ferrugineus)
全文: PDF (3039 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 红棕象甲(Rhynchophorus ferrugineus)是14种全国林业检疫性有害生物之一,其为害椰子树(Cocos nucifera)等棕榈科(Arecaceae)植物。本研究从患败血症的红棕象甲的体内分离出1株对红棕象甲幼虫具有杀虫活性的菌株Yel-8,通过形态学、16S rDNA、生理生化测定及药敏分析对该菌株进行鉴定。结果表明该菌株属于嗜虫耶尔森氏菌(Yersinia entomophaga)的一个新的菌株,其在L-阿拉伯糖、鼠李糖、木糖、蔗糖、七叶灵、纤二糖各项特征的分析中与嗜虫耶尔森氏菌的特征相同,仅在山梨醇的利用方面与嗜虫耶尔森氏菌相反,呈阴性。利用菌株Ye1-8进行生物测定表现出对红棕象甲具较好的杀虫活性(饲喂法和注射法的的半致死浓度(median lethal concentration, LC50)分别为3.184×108和5.1295×106 CFU/mg)。从菌株Ye1-8中分别克隆并表达了几丁质酶1(chitinase 1, chi1)基因和几丁质酶2 (chitinase 2, chi2)基因,结果表明Chi1和Chi2蛋白对于红棕象甲无杀虫活性,因此菌株Yel-8需要完整的毒素复合体才能发挥毒性。本研究为构建红棕象甲生防工程菌提供了新的材料。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
郭雅洁
韩红
廖启炓
林倩男
牟亚妮
翁小倩
邹双全
张飞萍
吴松青
关键词 红棕象甲嗜虫耶尔森氏菌分离鉴定杀虫活性    
Abstract:Rhynchophorus ferrugineus is a major quarantine pest that damages the coconut trees (Cocos nucifera) and other palm plants (Arecaceae). In this study, the strain Yel-8 was isolated from the corpse of R. ferrugineus, which had insecticidal activity against the larvae of R. ferrugineus which was suffered from sepsis and identified by morphological identification, 16S rDNA, physiological and biochemical tests, and drug susceptibility analysis, respectively. The results showed that Yel-8 was in line with the characteristic of Yersinia entomophaga in L-arabinose, rhamnose, xylose, sucrose, aesculin, and cellobiose metabolism, whereas opposed to Yersinia only in sorbitol metabolism. Therefore, this strain was identified as a new strain of Yersinia genus. The bioassay of Yel-8 against R. ferrugineus was performed using feeding and injection methods, and the median lethal concentration (LC50) of Yel-8 against R. ferrugineus was 3.184×108 and 5.1295×106 CFU/mg, respectively. The chitinase genes Chi1 and Chi2 were cloned from the Ye1-8 strain and the proteins were expressed for bioassay. The results showed that the Chi1 and Chi2 proteins had no insecticidal activity against R. ferrugineus alone, thus suggesting the necessity of complete toxin complex for toxicity. Yel-8 exhibited insecticidal activity may provide an ideal material for the construction of the biophytes against R. ferrugineus.
Key wordsRhynchophorus ferrugineus    Yersinia entomophaga    Isolation    Identification    Insecticidal activity
收稿日期: 2018-09-13      出版日期: 2018-11-20
基金资助:国家重点研发计划课题;国家自然科学基金项目;福建省科技厅自然科学基金项目;福建省林业科学研究项目;福建省科技计划项目;福建农林大学科研基金;福建农林大学林学院林学高峰学科项目;福建省大学生创新创业训练计划项目
通讯作者: 吴松青     E-mail: dabinyang@126.com
引用本文:   
郭雅洁 韩红 廖启炓 林倩男 牟亚妮 翁小倩 邹双全 张飞萍 吴松青. 嗜虫耶尔森氏菌Ye1-8的分离鉴定及其对红棕象甲的杀虫活性[J]. , 2018, 26(12): 2121-2131.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I12/2121
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn