Abstract：Abstract The meiotic maturation of spermatocyte in mammal is controlled by the maturation promotion factor (maturation promoting factor, MPF), a complex of CDK1(cyclin-dependent kinase, CDK1) and CyclinB1 proteins. In the present study, we selected 0,2,6,12,24-month-old sheep testis to investigate the expression and cellular localization of CDK1 and CyclinB1 in the sheep testes on different months . CDK1 and CyclinB1 genes expression levels in different months testis were analyzed by qRT-PCR(fluorescence quantitative Realtime PCR,qRT-PCR) and CDK1 and CyclinB1 localization in testis were detected by immunohistochemistry. The results showed that with month increasing, the expression of CDK1 and CyclinB1 mRNA were firstly decreased and then increased. CDK1 and CyclinB1 mainly localized in the spermatogonia, primary spermatocytes and secondary spermatocytes. CDK1 protein always expressed at an extremely low level throughout all stage of development. However, its expression quantity was relatively stable. On the other side, the monthly expression difference of CyclinB1 protein in sheep testis of were remarkable, the expression level was always higer than that of CDK1 protein. These results indicated that the expression of CDK1 and CyclinB1 mRNA and protein in sheep testes at different months were significant. And it may play an important role in sheep testis development.
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