Abstract：The reproductive performance and health status of alpha-1, 3 galactosyltransferase gene(GGTA1) knockout pigs(Sus scrofa) is the key to success of xenotransplantation. Here we examined the genotype, litter size, blood routine, blood biochemical indexs of our group's GGTA1 knockout(GTKO) Bama minipigs(BMP) to assess their reproductive capacity and health status. GTKO pigs were mated with GTKO or wild type BMPs. The GTKO BMP have been bred to F3 generation. Firstly, the typing of GGTA1 gene knockout was identified by sequencing PCR products. α-1,3-Gal which is the product of alpha-1,3 galactosyl transferase gene was detected by flow cytometry after mixing Fluorescein isothiocyanate-Griffonia simplicifolia Isolectin B4(FITC-GSIB4) and peripheral blood mononuclear cells(PBMC). Then, reproductive performance was evaluated by litter size. Health status was assessed by blood routine and blood biochemical indexes. The results showed that the inheritance of GGTA1 gene was followed the Mendelian law; Low fluorescence expression were detected in GGTA1 double knocked (GGTA1-/-) genotype. The litter size of gilt was 7.33±1.70, and the litter size of sow was 9.43±1.68, which had no significant difference in the fecundity with wild BMP Meanwhile, compared with wild-type BMP, no obvious difference were found in blood routine and blood biochemical indexes. In short, our results demonstrated that GTKO BMPs' family was established, with stable hereditary, normal fertility and healthy physiological indicators. The GGTA1-/- Bama minipigs which effectively solved the hyperacute rejection, it can be used as ideal donor for xenotransplantation research.
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