Abstract：In order to select atrazine-degrading consortium and strain resources, in the present research, by enrichment culture, a stable 4-member bacterial consortium NC1 was isolated from long-term use of atrazine surface corn-planted soil (Nancha area, Yichun City, Heilongjiang Province, China). The consortium NC1 was attenuated and plated on mineral salts medium agar plates, strains were repeatedly crossed vaccination on mineral salts medium agar plates for separation of pure culture, 4 bacterial isolates, named strain YJY1, YJY2, YJY3 and YJY5. Based on physiological and biochemical tests, and 16S rDNA gene sequence analysis, 4 bacterial strains were shown to belong to the Gram-negative species Enterobacter sp., Achromobacter xylosoxidans, Pseudomonas aeruginosa and Klebsiella pneumonia, respectively. These species were not stated previously as being capable of atrazine-degrading. By growth capacity measuring, consortium NC1, strain YJY5 could use atrazine as single nitrogen source for growth, and strain YJY1, YJY2 and YJY3 could not. Consortium NC1 and 4 strains could hardly grow in MSM with cyanuric acid as the sole nitrogen source. Consortium NC1 and strain YJY5 had a short lag period (8~10 h), then was logarithmic phase. Strain YJY5 was stagnate phase of 28~36 h, and consortium NC1 was still growing rapidly at 36 h and capable of degrading 100 mg/L atrazine to nontoxic cyanuric acid. Altogether this was a new combination of isolates in an atrazine- degrading consortium, and its degradation rate and the growth were both faster than that of the sole strain. The high atrazine degradation ability of the consortium NC1 showed good potential for atrazine biodegradation. This research will contribute toward a better understanding of the metabolic activities of atrazine-degrading consortium, which are generally considered to be responsible for atrazine mineralization in the natural environment. The results provide information and reference for the study on biodegradation of atrazine work.
[1]Johnson TA, Ellsworth TR, Hudson RJM, et al.2013. Diffusion Limitation for Atrazine Biodegradation in Soil[J]. Advances in Microbiology, 3(5):412-420.[J].Advances in Microbiology, 2013, 3(5):412-420[2]Ackerman F.2007. The economics of atrazine[J]. International Journal of Occupational and Environmental Health. 13(4):437-445.[J].International Journal of Occupational and Environmental Health, 2007, 13(4):437-445[3]李红梅, 张新建, 李纪顺, 等.2014. 阿特拉津降解菌SD41的分离鉴定及土壤修复[J]. 环境科学与技术. 37(4):38-41.[J].环境科学与技术, 2014, 37(4):38-41[4]陈建军, 何月秋, 祖艳群, 等.2010. 除草剂阿特拉津的生态风险与植物修复研究进展[J]. 农业环境科学学报. 29(1): 289-293.[J].农业环境科学学报, 2010, 29(1):289-293[5]Freitas L G, Singer H, Müller S R, et al.2008. Source area effects on herbicide losses to surface waters—A case study in the Swiss Plateau[J]. Agriculture Ecosystems & Environment. 128(3):177-184.[J].Agriculture Ecosystems & Environment, 2008, 128(3):177-184[6]Safe S.2005. Clinical correlates of environmental endocrine disruptors[J]. Trends Endocrinol Metab. 16(4):139-144.[J].Trends Endocrinol Metab, 2005, 16(4):139-144[7]Bianchi C L, Pirola C, Ragaini V, et al.2006. Mechanism and efficiency of atrazine degradation under combined oxidation processes[J]. Applied Catalysis B: Environmental. 64(1-2):131-138.[J].Applied Catalysis B: Environmental, 2006, 64(1-2):131-138[8]H, Asakura, and Matsuto T.2009. Experimental study of behavior of endocrine-disrupting chemicals in leachate treatment process and evaluation of removal efficiency[J]. Waste Management. 29(6): 1852–1859.[J].Waste Management., 2009, 29(6):1852-1859[9]董春香, 姜桂兰.2001. 除草剂阿特拉津生物降解研究进展[J]. 环境污染治理技术与设备. 2(3): 1-6.[J].环境污染治理技术与设备, 2001, 2(3):1-6[10]Cheyns K, Martin-Laurent F, Bru D, et al.2012. Long-term dynamics of the atrazine mineralization potential in surface and subsurface soil in an agricultural field as a response to atrazine applications[J]. Chemosphere. 86(10):1028–1034.[J].Chemosphere, 2012, 86(10):1028-1034[11]Souza M L D, Sadowsky M J, Wackett L P, et al.1999. Atrazine chlorohydrolase from Pseudomonas sp. strain ADP: gene sequence, enzyme purification, and protein characterization[J]. J Bacteriol. 181(16): 695.[J].J Bacteriol, 1999, 181(16):695-695[12]K S, N S, LP W, et al.2004. Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli[J]. Appl Environ Microbiol. 70(7): 4402–4407.[J].Appl Environ Microbiol, 2004, 70(7):4402-4407[13]Zhang Y, Cao B, Jiang Z, et al.2012. Metabolic ability and individual characteristics of an atrazine-degrading consortium DNC5[J]. J Hazard Mater. 237(17):376-381.[J].J Hazard Mater, 2012, 237(17):376-381[14]赵斌, 何绍江.2002. 微生物学实验[M]. 科学出版社, 北京. pp. 121-159.[15]George MG, Julia AB, Timothy GL.2004. Bergey’s Manual of Systematic Bacteriology. 2nded[M]. Springer Publishing Company, New York. pp. 209-211.[16]刘春光, 杨峰山, 卢星忠等.2010. 阿特拉津降解菌T3 AB1的分离鉴定及土壤修复[J]. 微生物学报. 50(12):1642-1650.[J].微生物学报, 2010, 50(12):1642-1650[17]王静, 李迎芳, 裴丽娟等.2013. 水中阿特拉津的自动固相萃取-高效液相色谱法测定[J]. 河南科学. 33(2):165-167.[J].河南科学, 2013, 33(2):165-167[18]Zhang Y, Jiang Z, Cao B, et al.2011. Metabolic ability and gene characteristics of Arthrobacter sp. strain DNS10, the sole atrazine-degrading strain in a consortium isolated from black soil[J]. International Biodeterioration & Biodegradation. 65(8):1140-1144.[J].International Biodeterioration & Biodegradation, 2011, 65(8):1140-1144[19]Behki R M, Khan S U.1986. Degradation of atrazine by Pseudomonas' N dealkylation and dehalogenation of atrazine and its metabolites[J]. J. Agric. Food Chem. 34(4):746-749. [J].J. Agric. Food Chem, 1986, 34(4):746-749[20]Q L, Y L, X Z, et al.2008. Isolation and characterization of atrazine-degrading Arthrobacter sp. AD26 and use of this strain in bioremediation of contaminated soil[J]. Journal of Environmental Sciences. 20(10):1226–1230.[J].Journal of Environmental Sciences, 2008, 20(10):1226-1230[21]Jinhua Wang, Lusheng Zhu, Qi Wang, et al.2014. Isolation and Characterization of Atrazine Mineralizing Bacillus subtilis Strain HB-6[J]. PLoS ONE. 9(9): e107270[J].PLoS ONE, 2014, 9(9):e107270-e107270[22]E T, H Z, SM N, et al.2000. Characterization of an atrazine-degrading Pseudaminobacter sp. isolated from Canadian and French agricultural soils[J]. Applied and Environmental Microbiology. 66(7): 2773–2782.[J].Applied and Environmental Microbiology, 2000, 66(7):2773-2782[23]Behki R.1993. Metabolism of the herbicide atrazine by Rhodococcus strains[J]. Applied and Environmental Microbiology. 59(6):1955-1959.[J].Applied and Environmental Microbiology, 1993, 59(6):1955-1959[24]C Y.2010. Atrazine degradation by a simple consortium of Klebsiella sp. A1 and Comamonas sp. A2 in nitrogen enriched medium[J]. Biodegradation. 21(1):97-105.[J].Biodegradation, 2010, 21(1):97-105[25]王志刚, 张颖, 郭火生等.2014. 阿特拉津降解菌Acinetobacter sp.DNS32对无机氮源的响应[J]. 微生物学通报. 41(8):1541-1546.[J].微生物学通报, 2014, 41(8):1541-1546
