Abstract：Thioredoxin (Trx) is a kind of highly conservative and low molecular weight proteins, which widely exists in plants, animals, bacteria and yeasts. It maintains the redox equilibrium of organisms, which regulates and controls biological signal transduction and other functions. This study cloned VvTrx gene from grape (Vitis vinifera) gDNA sequence (GenBank No. EU280166) of 989 bp and cDNA sequence (GenBank No. EU280164) of 561 bp and the open reading frame (ORF) was 381 bp, encoding 126 amino acids. The multiple sequence alignment and phyogenetix tree showed that the active site of VvTrx gene in Thompson Seedless (Vitis vinifera cv.) was WCIPS and belonged to the subgroup Ⅳ in Trx-h. Analysis of quantitative Real-time PCR(qRT-PCR) showed that, in different periods of the ovule development of both Thompson Seedless and Pinot Noir, the expression of VvTrx gene reached the highest level in 40 d after flowering. Besides, the difference between them was extremely significant (P＜0.001) and the expression of Thompson Seedless was about 5 times of Pinot Noir. After that, the VvTrx gene was cloned into the prokaryotic expression vector of pET-32a(+) to construct pET-32a(+)/VvTrx, and a 30 kD solubility fusion protein expressed in the Escherichia coli BL21 through isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. The study showed that the VvTrx gene had a certain relation to the process of seedless grape ovules abortion, which provides the research directions to the molecular mechanism of VvTrx gene in seedless grape embryo abortion process and the breeding of seedless grape.
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