Abstract：Cytochrome P450 enzymes (CYPs) are involved in many physiological functions in insects, such as the metabolism of signal molecules, adaption to host plants and insecticide resistance. Species of Lepidoptera, which include the most disruptive agricultural pests, rank only secondly to those of Coleoptera. On basis of the draft genome sequence of lepidopteran insect, Manduca sexta L., a genome-wide analysis of CYPs was performed and 110 CYP-related sequences were obtained, which could be classified into 29 families according to standard nomenclature, a lot of which were tandemly arranged on chromosomes. We furtherly compared the CYP-related sequences with those of monarch butterfly (Danaus plexippus L.) and the model insect silkworm (Bombyx mori L.). The result revealed that there were 12 pairs of orthologs in CYP2 and mitochondrial clans, whereas many CYPs in CYP3 and CYP4 clans were present with species-speci?c expansions. There were more orthologous CYP pairs among lepidopteran insects in comparsion with those between lepidopteran insects and the other insect orders. Many orthologous groups of paralogous genes could be also distinguished from the phylogenetic tree in spite that a high frequency of species-specific expansion, relatively few different CYP families and subfamiles were represented among different lepidopteran insects. Extensive synteny of CYPs was also maintained in the different lepidopteran insect genomes. The insights obtained from comparative genomic analysis will greatly facilitate researches on all lepidopteran CYPs, and in particularly on selective targets for innovative pest management.
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