Abstract：Abstract: In the present study, the domestic yak (B.Grunniens) metallothionein-I and Ⅲ cDNA 3′-end full length sequences (331bp, 378bp) were amplified and cloned by RT-PCR and 3′-RACE using the primer YMT-ⅠSP, YMT-ⅢSP and M13 Primers M4, in which included MT-I and MT-Ⅲ cDNA coding sequences (183bp, 207bp) and AATAAA and Poly（A）in the end. The cDNA sequences of MT-I and MT-Ⅲ were subjected to Blastn searching in CBI and the results showed that the nucleotide sequences of yak MT-I and MT-Ⅲ between different species mammal are comparatively conservative. The yak cDNA sequence coding for yak MT-I protein is composed of 61 amino acids, including 20 Cysteine. There are lots of conserved tripetide, such as C-X-C, C-C-X-C-C, C-X-X-C, etc.. The yak cDNA sequence coding for yak MT-Ⅲ protein is composed of 68 amino acids, including 19 Cysteine, which has the same conserved tripetide with MT-Ⅰ, and MT-Ⅲ has also its own particular conserved tripetide, such as T, CPCP,GEGAEA, etc.. They are all comparatively conservative in their evolution.
刘斌 吴建平 马彬云 高凤芹. 牦牛MT-I/Ⅲ基因cDNA 3′末端全长的克隆与序列分析[J]. , 2008, 16(4): 0-.
Bin LIU Jian-ping WU Bin-yun MA Feng-qin GAO. Cloning of 3′-end full length of cDNA encoding for MT-I/Ⅲ and analyzing of the sequence in Yak. , 2008, 16(4): 0-.
