A Quick Molecular Identification for the Species Sources of Animal Fibres
XIANG Hai1, ZHU Piao1, ZHANG Xing1,2, YANG Liu1,2, ZHAO Xing-Bo1,2,*
1 Guangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, College of Life Science and Engineering, Foshan University, Foshan 528231, China; 2 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Abstract：Identification of species origin of animal fibres is important in textile inspection work. In this study, a universal primer set for yak (Bos grunniens), camel (Camelus dromedarius and Camelus bactrianus), sheep (Ovis aries), goat (Capra hircus), cattle (Bos taurus) and pig (Sus scrofa) were designed according to their mitochondrial genomes. Moreover, species-specific primer sets for yak, camel, sheep and goat as well as a universal primer set for goat and sheep were also generated. After that, several DNA extraction methods were compared to find out the most optimal method for animal wool shaft fiber. Then the preservation status of samples were determined rapidly and examined whether it was mixed accurately by sequencing the amplicon of the universal primer set. By combining the species-specific primers amplification and electrophoresis detection, we are able to identify the animal species sources of wool shaft fibers with limit time and cost. In this study, an accurate, rapid and simple molecular identification system was established to distinguish the animal species sources of wool shaft fibers, which provides a technical reference for the quality control of textile products.
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