Abstract：Abstract: Pathogenic mutant isolates 9 from the greenhouse and 5 from the International Rice Research Institute Blast Nursery, respectively, were identified by using molecular techniques with Rep-PCR of the two primer pairs, Pot2-1/Pot2-2 and PMC1-2/PMC1-3, RAPD of 177 primers and RFLP of MGR586. The results indicated that the 9 mutants from greenhouse had almost identical DNA band patterns and their original isolates lost 1~3 bands for 7 mutants, but no changes for the other 2 mutants. The 5 mutants from the nursery, which defeated resistance genes Pi-1 and Pi-2, originated from the isolates with avirulence gene Avr-1 in the field, not from outside by genetic shift based on their pathotypes and DNA band patterns. Therefore, it was concluded that the mutation was a main evolution force for Magnaporthe grisea.
何月秋1，2 Hei LEUNG2 Robert S. ZEIGLER2 唐文华3. 稻瘟病菌突变菌株的分子鉴定[J]. , 2003, 11(1): 89-93.
He Yueqiu1,2 Hei LEUNG2 Robert S. ZEIGLER2 Tang Wenhua3. Molecular Identification of the Mutant Isolates of Magnaporthe grisea. , 2003, 11(1): 89-93.
