Abstract：The myogenic factor 5 (MYF5) gene is considered to be an important gene affecting muscle growth and development. The aim of this study was to investigate the effect of genetic variation of MYF5 gene on growth traits in sheep (Ovis aries), in order to provide effective molecular genetic markers for the breeding of new breeds (lines) of high quality meat sheep. Three SNP loci (124510044, 124509770 and 124507708) with polymorphism and significant interbreed differences were screened. The selected loci were examined by flight mass spectrometry in 383 sheep from the 3 crosses and correlated with growth traits at birth and 3 months of age. The results showed that locus 12451044 detected 3 genotypes, which were moderately polymorphic (0.25 ≤PIC< 0.50) in all populations; locus 124509770 detected 3 genotypes, which were low polymorphic (PIC< 0.25) in the H1 generation population and moderately polymorphic (0.25 ≤PIC< 0.50) in the other populations. Only one genotype deletion was detected at locus 124507708. In the H2 generation population, individuals with CT genotype at locus 124510044 had significantly higher body height at 3 months of age than TT genotype (P<0.05), and individuals with CG genotype at locus 124509770 had significantly higher body height at 3 months of age than GG genotype (P<0.05); individuals with GG genotype had significantly higher chest circumference at 3 months of age than CC genotype, regardless of generation (P<0.05 ). 124510044 and 124509770 loci were complete linkage disequilibrium, producing 4 haplotypes and 5 diploids; individuals with diploid H4H4 had significantly higher chest circumference at birth and at 3 months of age than H1H1 (P<0.05). The results of this study showed that genetic variation in the MYF5 gene has an effect on growth traits in sheep, and the loci 124510044 and 124509770 can be used as potential candidate genetic markers for growth and development and meat production performance in meat sheep.This study provides an important reference for the MYF5 gene in the selection and breeding of meat sheep, and provides a theoretical basis for molecular marker-assisted breeding in meat sheep.
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