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2025年4月3日 星期四
农业生物技术学报  2022, Vol. 30 Issue (3): 425-433    DOI: 10.3969/j.issn.1674-7968.2022.03.002
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
过表达GbRac1基因增强大豆对疫霉根腐病的抗性
王钟伟1,2, 张原宇2, 刘东波1,2, 牛陆2, 杨静2, 杨向东2, 仲晓芳2,*
1 吉林师范大学 生命科学学院, 四平 136000;
2 吉林省农业科学院 农业生物技术研究所/吉林省农业生物技术重点实验室,长春 130033
Over-expression of GbRac1 Gene Enhances the Tolerance to Phytophthora Root and Stem Rot in Soybean (Glycine max)
WANG Zhong-Wei1,2, ZHANG Yuan-Yu2, LIU Dong-Bo1,2, NIU Lu2, YANG Jing2, YANG Xiang-Dong2, ZHONG Xiao-Fang2,*
1 School of Life Science, Jilin Normal University, Siping 136000, China;
2 Agricultural Biotechnology Institute/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
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摘要 疫霉根腐病(Phytophthora root and stem rot, PRR)是影响大豆(Glycine max)生产的毁灭性病害之一,可造成大豆产量损失10%~50%。转基因技术为抗疫霉根腐病大豆品种培育提供了有效手段。小G蛋白(small GTPases)作为植物信号转导中的“分子开关”,不仅在植物生长发育过程中参与调控植物对外界环境刺激的响应,同时在植物防卫反应的建立中也发挥着重要的作用。本研究将海岛棉(Gossypium barbadense)小G蛋白编码基因Rac1 (Gossypium barbadense Rac family small G protein 1, GbRac1)导入栽培大豆品种'Williams 82'中。Southern blot和逆转录PCR (reverse transcription PCR, RT-PCR)检测结果表明,外源基因已整合至大豆基因组中且能够稳定表达。T3和T4代转基因大豆株系的PRR抗性鉴定是采用下胚轴接种法接种疫霉菌1号生理小种,接种7 d后,对照品种'Williams 82'出现叶片萎蔫和植株死亡现象,而多数转基因植株则表现正常,其接种存活率为70.68%~90.63%,而对照存活率仅为40.74%~50.85%。在未接种疫霉菌1号生理小种的生长条件下,过表达GbRac1基因的转基因大豆株系的主要农艺性状与受体'Williams 82'相比没有显著差异。以上研究结果的获得,说明过表达GbRac1基因显著增强了转基因大豆对疫霉根腐病的抗性,且对其他表型性状未产生非特异性的影响。本研究为进一步拓宽大豆抗性遗传基础,培育抗疫霉根腐病大豆新品种提供了基础资料。
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王钟伟
张原宇
刘东波
牛陆
杨静
杨向东
仲晓芳
关键词 大豆疫霉根腐病疫霉菌棉花小G蛋白Rac1 (GbRac1)抗病性    
Abstract:Phytophthora root and stem rot (PRR) represents one of the destructive diseases in soybean (Glycine max) production, generally causing 10%~50% yield loss every year. Transgenic biotechnology provides an effective strategy for preventing and controlling the occurrence of PRR. The small G protein, known as the 'molecular switch', is not only involved in the process of regulating the adaptation of plants to external environmental stimuli during plant growth and development, but also participates in the establishment of plant defense responses. In this study, Gossypium barbadense Rac family small G protein 1 gene (GbRac1) was introduced into the soybean cultivar 'Williams 82'. Integration and expression of the foreign gene in transgenic soybean plants were confirmed by Southern blot and reverse transcription-PCR (RT-PCR) analysis. The PRR tolerance of the T3 and T4 generations of transgenic soybean lines was evaluated using a hypocotyl inoculation method. At 7 d after inoculation with Phytophthora sojae race 1, typical PRR symptoms such as wilting leaves and plant death were observed in the non-transformed plants. In contrast, most of transgenic plants showed no visible symptoms and developed normally with the survival rate of 70.68%~90.63%, higher than that of the control plants (40.74%~50.85%). However, no significant differences in agronomic traits were observed between the transgenic and non-transformed plants in the field conditions when no P. sojae inoculation involved. Taken together, these results demonstrated that over-expression of GbRac1 significantly enhanced the tolerance of the transgenic soybean to PRR without negative consequences on the agronomic performance. This results also pave the way for broadening disease resistance in soybean and development of the PRR-resistant soybean cultivars.
Key wordsSoyean    Phytophthora root and stem rot    Phytophthora sojae    Gossypium barbadense Rac family small G protein 1 (GbRac1)    Disease resistance
收稿日期: 2021-06-02     
ZTFLH:  S336  
基金资助:吉林省农业科技创新工程项目(CXGC2021TD015)
通讯作者: *xfzhong649@163.com   
引用本文:   
王钟伟, 张原宇, 刘东波, 牛陆, 杨静, 杨向东, 仲晓芳. 过表达GbRac1基因增强大豆对疫霉根腐病的抗性[J]. 农业生物技术学报, 2022, 30(3): 425-433.
WANG Zhong-Wei, ZHANG Yuan-Yu, LIU Dong-Bo, NIU Lu, YANG Jing, YANG Xiang-Dong, ZHONG Xiao-Fang. Over-expression of GbRac1 Gene Enhances the Tolerance to Phytophthora Root and Stem Rot in Soybean (Glycine max). 农业生物技术学报, 2022, 30(3): 425-433.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.03.002     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I3/425
 
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