Abstract：The yeast two-hybrid (Y2H) system is one of the most effective molecular biology methods to identify protein-protein interactions. Y2H system has played an important role in proteomics, functional genomics, pathology and other research fields. Due to the success of Y2H, many derivative technologies based onY2H have been developed in recent years. The principle of Y2H, one-hybrid system, three-hybrid system, reverse two-hybrid system, cytosolic yeast two-hybrid system and other derivative systems were reviewed in this paper. The application of Y2H in high-throughput screening, quantitative analysis of protein interaction, protein interactome, pathogenic mechanism, search for new drug targets, and agriculture were introduced as well. The prospect of Y2H was also brought out in this article. This review will help researchers comprehend the principles and development process of Y2H. It can also help researchers understand the application scope of Y2H. This review would provide references for those who need to use Y2H to study protein-protein interactions.
沈竹, 曹勤红. 酵母双杂交及其衍生技术应用研究进展[J]. 农业生物技术学报, 2022, 30(12): 2425-2433.
SHEN Zhu, CAO Qin-Hong. Research Progress on Application of Yeast Two Hybrid System and Y2H-derivated Techniques. 农业生物技术学报, 2022, 30(12): 2425-2433.
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