联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月5日 星期六
农业生物技术学报  2021, Vol. 29 Issue (3): 472-480    DOI: 10.3969/j.issn.1674-7968.2021.03.007
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
甘蔗SsCBL3基因克隆与表达分析
凌秋平, 曾巧英, 周文灵, 敖俊华, 黄莹, 吴启华, 沈大春*
广东省科学院 生物工程研究所/广东省现代农业产业技术研发中心(资源环境与农产品安全),广州 510316
Cloning and Expression Analysis of SsCBL3 in Sugarcane (Saccharum officinarum)
LING Qiu-Ping, ZENG Qiao-Ying, ZHOU Wen-Ling, AO Jun-Hua, HUANG Ying, WU Qi-Hua, SHEN Da-Chun*
Institute of Bioengineering, Guangdong Academy of Sciences/Guangdong Modern Agricultural Technology Research and Development Center (Resources and Environment and Agricultural Product Safety), Guangzhou 510316, China
全文: PDF (5003 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 类钙调神经素B亚基蛋白(calcineurin B-like proteins, CBLs)是解码钙信号的一类重要蛋白,在植物遭受非生物胁迫过程中发挥重要调节作用。本研究以甘蔗(Saccharum officinarum) '新台糖22号'为实验材料,基于基因转录结果,采用反转录PCR (reverse transcription-PCR, RT-PCR)从低钾胁迫甘蔗根系中克隆得到SsCBL3基因(GenBank No. MN850491)。该基因包含1个长度为678 bp的开放阅读框,预测可编码225个氨基酸。对SsCBL3蛋白的序列进行生物信息学分析,显示蛋白分子量为25.767 47 kD,理论等电点(isoelectric point, PI)为4.82,预测其属于酸性蛋白。分析SsCBL3的蛋白结构,结果显示,其含有3个的EF-hand (elongation factor hand)结构域和1个与类钙调磷酸酶B互作蛋白激酶(CBL-interacting protein kinase, CIPK)互作的FPSF位点。系统进化树分析结果显示,甘蔗SsCBL3与禾本科作物的亲缘关系较近,与玉米(Zea mays)的亲缘关系最近。实时荧光定量PCR检测结果发现,SsCBL3基因在低氮、低磷、低钾、干旱、盐和脱落酸(abscisic acid, ABA)胁迫过程中的表达均发生变化,并且胁迫时间的不同也会造成基因表达差异。其中,盐和干旱胁迫能够诱导SsCBL3基因强烈表达。qRT-PCR结果说明SsCBL3基因可能在甘蔗遭受逆境胁迫下发挥重要的调控作用。本研究为培育抗逆甘蔗品种提供理论基础。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
凌秋平
曾巧英
周文灵
敖俊华
黄莹
吴启华
沈大春
关键词 甘蔗类钙调神经素B亚基蛋白基因3 (CBL3)基因克隆基因表达    
Abstract:Calcineurin B-Like proteins (CBLs) are a kind of important protein which can involve in decoding calcium signals in plants. They play important role in plant response to various stresses. The cDNA of SsCBL3 (GenBank. MN850491) cloned from sugarcane (Saccharum officinarum) cultivar 'ROC22' under low potassium stress by reverse transcription-PCR (RT-PCR). SsCBL3 contained a complete open reading frame of 678 bp encoding a protein with 225 amino acids. The sequence features of SsCBL3 analyzed by bioinformatics. The molecular weight of SsCBL3 was 25.767 47 KD and the isoelectric point was 4.82, which was an acidic protein. The structure of SsCBL3 protein was analyzed showed that it contained 3 EF-hand functional domains and 1 FPSF motif which interacted with CIPK kinases. Evolutionary analysis further revealed that SsCBL3 has high homology to the CBL3 from gramineous crop, and had the closest relationship with Zea may. The result of qRT-PCR showed that the expression of SsCBL3 was changed under low nitrogen, low phosphorus, low potassium, drought, salt and ABA stresses. Moreover, the different stress times also change expression of SsCBL3. The expression of SsCBL3 strongly induced by drought or salt stresses. The results of qRT-PCR indicated that SsCBL3 gene may play an important regulatory role in sugarcane under stress. The results of research provides a theoretical basis for cultivating sugarcane varieties with stress-resistant.
Key wordsSugarcane    Calcineurin B-Like proteins 3 (CBL3)    Gene cloning    Gene expression
收稿日期: 2020-06-28      出版日期: 2021-03-01
ZTFLH:  S566.1  
基金资助:国家自然科学基金(41907130); 广东省科学院发展专项(2019GDASYL-0103033); 国家农业农村部糖料产业技术体系(CARS-170203); 广东省甘蔗改良与生物炼制重点实验室开放运行基金(2017B030314123)
通讯作者: *shdach@163.com   
引用本文:   
凌秋平, 曾巧英, 周文灵, 敖俊华, 黄莹, 吴启华, 沈大春. 甘蔗SsCBL3基因克隆与表达分析[J]. 农业生物技术学报, 2021, 29(3): 472-480.
LING Qiu-Ping, ZENG Qiao-Ying, ZHOU Wen-Ling, AO Jun-Hua, HUANG Ying, WU Qi-Hua, SHEN Da-Chun. Cloning and Expression Analysis of SsCBL3 in Sugarcane (Saccharum officinarum). 农业生物技术学报, 2021, 29(3): 472-480.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.03.007     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I3/472
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn