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2025年4月5日 星期六
农业生物技术学报  2020, Vol. 28 Issue (8): 1441-1449    DOI: 10.3969/j.issn.1674-7968.2020.08.011
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
酉州乌羊LncRNA XLOC_15448的组织表达及其在黑色素沉积过程中互作分子的预测
付琳, 任航行, 王高富, 蒋婧, 李杰, 孙晓燕, 刘良佳, 周鹏, 张丽*
重庆市畜牧科学院/重庆市山羊工程技术研究中心,重庆 402460
Tissue Expression of LncRNA XLOC_15448 in Youzhou Dark Goat (Capra hircus) and Prediction of Its Interaction Molecules in Melanoma Pathogenesis
FU Lin, REN Hang-Xing, WANG Gao-Fu, JIANG Jing, LI Jie, SUN Xiao-Yan, LIU Liang-Jia, ZHOU Peng, ZHANG Li*
1 Chongqing Academy of Animal Sciences/Chongqing Engineering Research Center for Goat, Rongchang 402460, China
全文: PDF (2201 KB)   HTML (1 KB) 
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摘要 长链非编码RNA (long noncoding RNA, lncRNA)在哺乳动物的基因组中被广泛转录,能够通过相应的micRNA和靶基因发挥其生物学功能,并通过生物信息学预测和实验验证相结合方法,快速、有效地获取并筛选LncRNA靶基因。为探究酉州乌羊(Capra hircus) LncRNA XLOC_15448的组织表达谱及其在黑色素沉积过程的靶基因,本研究以酉州乌羊和酉州本地白山羊为实验对象,检测LncRNA XLOC_15448在各组织中的表达,比较其在乌皮与非乌皮中的表达差异;同时,检测LncRNA XLOC_15448在小鼠(Mus musculus)皮肤黑色素瘤细胞细胞系B16-F10中的表达特性;最后,通过靶micRNA和靶基因预测,构建lncRNA-miRNA-mRNA调控网络关系。研究结果显示,LncRNA XLOC_15448在酉州乌羊不同体组织中广泛表达,其中在心脏中的表达量最高,其次为肌肉、大脑、皮肤、肝、肾、肺、脾脏组织;其中在酉州乌羊皮肤中的表达量显著高于酉州本地白山羊皮肤组织;体外实验研究发现,随着B16-F10细胞的增殖,LncRNA XLOC_15448表达量呈递增趋势;同时,软件预测显示,LncRNA XLOC_15448可能通过靶向miR-182-5p调控小眼畸形相关转录因子(microphthalmia-associated transcription factor, MITF)基因的表达。基于此,本研究表明LncRNA XLOC_15448可能通过LncRNA-miRNA-mRNA网络参与了酉州乌羊皮肤黑色素细胞的生长及黑色素的沉积,这为进一步深入探究山羊皮肤着色相关的lncRNA调控理论的研究提供了基础资料。
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作者相关文章
付琳
任航行
王高富
蒋婧
李杰
孙晓燕
刘良佳
周鹏
张丽
关键词 酉州乌羊长链非编码RNA (LncRNA)组织表达B16细胞黑色素    
Abstract:Long noncoding RNA (LncRNA) was widely transcribed in the mammalian genome. LncRNA can play biological functions through the corresponding micRNA and target genes. By combining bioinformatics prediction and experimental verification, the LncRNA target genes can be rapidly and efficiently obtained and screened. To further explore the expression characteristics and predict its interaction molecules in melanoma pathogenesis of LncRNA XLOC_15448, the tissue expression profile LncRNA XLOC_15448 was detected using qRT-PCR method in different tissues of Youzhou dark goat (Capra hircus) and Youzhou blank goat (Capra hircus), as well as the LncRNA XLOC_15448 expression characteristics were detected in B16 cells at different developmental stages. The target genes were also predicted using miRBase, TargetScan7.2, miRTarBase, RNA22 version 2.0, and RNAhybrid2.2, and finally the lncRNA-miRNA-mRNA regulatory network was constructed in Capra hircus. The results demonstrated that LncRNA XLOC_15448 was widely expressed in different tissues of the goat, especially with the highest expression in the heart and lowest level in spleen, and it was also widely expressed in muscle, brain, skin, liver, kidney, lung and spleen. Comparison analysis illustrated that the skin LncRNA XLOC_15448 was expressed significantly higher in Youzhou dark goat than that of Youzhou blank white goat, and that the expression pattern of the lncRNA was increasing during the B16 cell proliferation. Bio-information analysis indicated that LncRNA XLOC_15448 might regulate gene expression of MITF (microphthalmia-associated transcription factor) by targeting to miR-182-5p. It was speculated that there may be a lncRNA-miRNA-mRNA network which modulate the dermal melanogenesis and melanin deposition in Youzhou dark goat, which would provide the basic data for further research on the regulatory mechanisms of lncRNAs related to goat skin pigmentation.
Key wordsYouzhou dark goat    Long noncoding RNA (LncRNA)    Tissue expression    B16 cell    Melanin
收稿日期: 2019-08-28     
ZTFLH:  S828.2  
基金资助:国家自然科学基金(31672393);重庆市2018年科研机构绩效激励引导专项(cstc2018jxjl0039);中央引导地方科技发展专项资金地方科技创新项目示范类(19201)
通讯作者: *zhangli10078244@163.com   
引用本文:   
付琳, 任航行, 王高富, 蒋婧, 李杰, 孙晓燕, 刘良佳, 周鹏, 张丽. 酉州乌羊LncRNA XLOC_15448的组织表达及其在黑色素沉积过程中互作分子的预测[J]. 农业生物技术学报, 2020, 28(8): 1441-1449.
FU Lin, REN Hang-Xing, WANG Gao-Fu, JIANG Jing, LI Jie, SUN Xiao-Yan, LIU Liang-Jia, ZHOU Peng, ZHANG Li. Tissue Expression of LncRNA XLOC_15448 in Youzhou Dark Goat (Capra hircus) and Prediction of Its Interaction Molecules in Melanoma Pathogenesis. 农业生物技术学报, 2020, 28(8): 1441-1449.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2020.08.011     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I8/1441
 
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