Abstract：Endometritis of cows (Bos taurus) has a significant impact on the fertility of dairy cows and restricts the development of dairy industry. Postpartum bacterial infection is the leading cause of endometritis. The main pathogenic bacteria isolated clinically are Escherichia coli and Staphylococcus aureus. In this study, bovine endometrial epithelial cells (BEECs) were cultured in vitro by two-step enzymatic digestion, and were infected with E.coli and S.aureus at different concentrations with corresponding multiple of infection (MOI). The TUNEL kit was used to detect apoptosis induced by the infection, and the mRNA levels of apoptosis-related genes Bax, Caspase-3 and Bcl-2 were detected by qRT-PCR after 12 h of the infection. The immunocytochemical identification showed that marker proteins (vimentin and keratin 18) of endometrial epithelial cells were positively stained. In E.coli and S.aureus infection groups, the apoptosis rate was the highest at 107 CFU/mL; E.coli and S.aureus infection induced increased expression of Bax and Caspase-3 (highest at 106 and 107 CFU/mL, respectively), and Bcl-2 expression decreased (lowest at 107 CFU/mL). This study indicated that E. coli and S. aureus could cause endometrial epithelial cell apoptosis, which might provide a reference for related mechanism researches on cow endometritis.
朱辰飞, 潘阳阳, 何翃闳, 赵凌, 王伟, 王彦雄, 崔燕, 余四九. 大肠杆菌与金黄色葡萄球菌感染对BEECs细胞凋亡相关因子表达的影响[J]. 农业生物技术学报, 2020, 28(6): 1004-1011.
ZHU Chen-Fei, PAN Yang-Yang, HE Hong-Hong, ZHAO Ling, WANG Wei, WANG Yan-Xiong, CUI Yan, YU Si-Jiu. Effects of Escherichia coli and Staphylococcus aureus Infection on the Expression of Apoptosis-related Factors in BEECs. 农业生物技术学报, 2020, 28(6): 1004-1011.
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