Diversity Study on Ectomycorrhizal Fungi from Fagaceae Roots in Russula Nature Reserve Area in Fujian
YU Wen-Ying1, HU Hong-Li2, BAO Jian-Dong1, PENG Ming-Hui2, LU Guo-Dong2,*
1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract：To protect and rationally utilize Russula resources and investigate the regulation of protective picking on the structure of ectomycorrhizal fungi in Fagaceae symbiotic host of mushroom Russula could provide a theoretical basis to improve Russula yield increasing in Natural Russula forest. In present study, using morphology analysis and ribosomal DNA internal transcribed spacer (rDNA ITS) sequencing, the dominant species was identified as Russula griseocarnosa in Fujian, while in some part of eastern Fujian, the major species was identified as R. rosea. In addition, the combination of morphology and rDNA ITS sequencing were used to compare the differences in the composition and abundence of ectomycorrhizal communities in Fagaceae symbiotic tree caused by non-protective and protective picking (that is, the last stubble of the previous year (about late August) all picked and not picked) in a Russula nature reserve area in Jianou, Fujian. A total of 34 OTUs (operational taxonomic units) was identified in ectomycorrhizal fungi from root tips of Fagaceae. Among them, 4 species, 9 genus with unknown species, 3 family with unknown genus and 1 order with unknown family were identified. The core communities were Lactarius, Thelephoraceae, Russula and Cortinarius. The protective picking of Russula in the last crop increased the abundance of Russula and Thelephoraceae in the second year, while the abundance of Lactarius decreased. The present study clarified the types of mycorrhizal fungi and found different harvesting of Russula in the last stubble could affect the symbiosis rate of Russula with the Fagaceae and flora structure of Fagaceae roots in the next year, which could provide a reference for the artificial promotion of Russula production as well as restoration and transformation of the Russula protected forest ecosystem.
余文英, 胡红莉, 鲍坚东, 彭明慧, 鲁国东. 福建正红菇保护林中壳斗科外生菌根真菌多样性研究[J]. 农业生物技术学报, 2020, 28(5): 771-783.
YU Wen-Ying, HU Hong-Li, BAO Jian-Dong, PENG Ming-Hui, LU Guo-Dong. Diversity Study on Ectomycorrhizal Fungi from Fagaceae Roots in Russula Nature Reserve Area in Fujian. 农业生物技术学报, 2020, 28(5): 771-783.
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