1 College of Agronomy and Biotechnology, Hebei Normal University of Science & Technology, Qinhuangdao 066000, China;
2 College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314000, China;
3 Jiaxing Second Hospital, Jiaxing 314000, China
Abstract：C1q tumor necrosis factor-related protein 6 (CTRP6) differentially regulates the metabolism of porcine intramuscular and subcutaneous fat, thus it has potential value in high quality lean pig (Sus scrofa) breeding. However, the effect of CTRP6 deficiency on animal health is still unknown. CTRP6 knock-out (KO) mice (Mus musculus) was constructed, and litter size, body weight, food intake, behavioristics of stressful situations, tissue sections, related physiological markers and gene expression were analyzed. The purpose is to explicit effects of CTRP6 deficiency on mice health. The results showed that average litter size was 5.4±0.3 in KO mice and 5.7±0.7 in wild-type (WT) mice. The survival rate was (92.1±3.2)% in KO mice and (93.1±4.5)% in WT mice. Two genotypes showed no significant difference. Weight analysis showed that the body weight of 8-week-old KO and WT mice were (25.08±0.34) and (28.34±1.06) g, respectively. The body weight of KO mice was significantly lower than that of WT mice (P<0.05), so does the weight of white adipose tissue (P<0.05). However, two genotypes showed no difference in average daily food intake. In tail suspension test and forced swimming test, the immobility time of KO mice was longer than that of WT mice, which indicates that KO mice were inclined to give up struggling under pressure conditions. In open field test, the number of crossing scores in KO mice was significantly less than in WT mice, which suggests that KO mice lack of interests in exploring a new environment. In sucrose preference test, no difference was observed between 2 genotypes in the sucrose preference. In morris water maze experiment, KO and WT mice exhibited similar learning ability in the first 5 days of place navigation test, but the frequency per min of passing through the plague position was less in KO mice than in WT mice on the 6th day of spatial probe test, which indicated the memory ability of KO mice was decreased. Further analysis of tissue section and inflammatory marker genes in brain were conducted and KO mice exhibited a higher level of inflammation than WT mice. The expression of memory related genes FBJ osteosarcoma oncogene (c-fos) in hippocampus was analyzed and it was down-regulated in KO mice. In conclusion, CTRP6 deficiency has no significant effect on mice litter size and food intake, but it reduces the body weight and adipose accumulation of mice. A negative impact on stress tolerance, interests of exploration and memory ability are detected, which may be related to the enhancement of brain inflammation. This study provides a certain theoretical basis for the further application of CTRP6 in high-quality lean pig breeding production. This study provides a theoretical basis for further revealing the function of ctrp6 gene.
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