The Characteristics of LH and PRL Hormones Changes in Small Tail Han Sheep (Ovis aries) and Sunite Sheep from Short-photoperiod to Long-photoperiod
XIA Qing1, *, ZHANG Jin-Long2, *, DI Ran1, HE Xiao-Yun1, ZHANG Xiao-Sheng2, WANG Xiang-Yu1, HU Wen-Ping1, LIU Qiu-Yue1, **, CHU Ming-Xing1, **
1 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture / Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2 Tianjin Institute of Animal Science, Tianjin 300381, China
Abstract：The majority of sheep (Ovis aries) in China are seasonal estrus, and the trait of estrus is regulated by hypothalamic-pituitary-gonadal axis related hormones. For exploring the secretion changes of luteinizing hormone (LH) and prolactin (PRL) in year-round estrous Small Tail Han (STH) sheep and seasonal estrous Sunite sheep at different photoperiods can contribute to understand the molecular mechanism of seasonal estrus in the term of hormone. In this study, the ovariectomized Small Tail Han sheep and Sunite sheep (SNT)were raised in light-controlled sheep house, keeping in short-photoperiod (SP) condition for 42 d, then transformed to long-photoperiod (LP) condition for 42 d, and jugular vein blood was collected in different light conditions. Radioimmunoassay was used to detect the concentration of LH and PRL in 2 breeds of sheep at different photoperiods. The results showed that the concentration of PRL in the blood of Sunite sheep was lower in short-photoperiod than that in long-photoperiod, and it was basically stable in different photoperiods in Small Tail Han sheep. It was significantly higher in Small Tail Han sheep than that in Sunite sheep at SP7 and SP21 (P<0.05). The concentration of PRL in Small Tail Han sheep was significantly lower than that in Sunite sheep after LP28 (P<0.05). The concentration of LH in the blood of Small Tail Han sheep was basically stable in different photoperiods. The concentration of LH in Sunite sheep was significantly lower than that in Small Tail Han sheep at SP21 (P<0.05). In summary, the change of PRL concentration in long-photoperiod and short-photoperiod was an important factor leading to the change of estrus status in seasonal estrus sheep. It was suggested that the concentration of PRL in long-photoperiod was high in Sunite sheep, which might weaken the LH response to gonadotropin-releasing hormone (GnRH) in the pituitary of the Sunite sheep, further to inhibit the follicular maturation and ovulation, and provide basic data for further exploration of the seasonal estrus mechanism of sheep in terms of hormones.
夏青, 张金龙, 狄冉, 贺小云, 张效生, 王翔宇, 胡文萍, 刘秋月, 储明星. 小尾寒羊和苏尼特羊从短光照到长光照过程中促黄体素和催乳素的变化特征[J]. 农业生物技术学报, 2020, 28(3): 483-489.
XIA Qing, ZHANG Jin-Long, DI Ran, HE Xiao-Yun, ZHANG Xiao-Sheng, WANG Xiang-Yu, HU Wen-Ping, LIU Qiu-Yue, CHU Ming-Xing. The Characteristics of LH and PRL Hormones Changes in Small Tail Han Sheep (Ovis aries) and Sunite Sheep from Short-photoperiod to Long-photoperiod. 农业生物技术学报, 2020, 28(3): 483-489.
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