Study on the Function of TabZIP3 in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina
LIU Na1,2,3, SUN Tian-Jie1,2,3, CHEN Yan1,2,3, HOU Chun-Yan1,2,3*, HAN Sheng-Fang1,2,3, WANG Dong-Mei1,2,3*
1 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China; 2 College of Life Sciences, Hebei Agricultural University, Baoding 071000, China; 3 Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, China
Abstract：Transcription factors play important roles in the response of plants to various stresses. The transcription factor family of bZIP (basic domain leucine zipper) is involved in the defense response against pathogen infection. In this study, the expression pattern of TabZIP3 (Genbank No. BAD97365.1), a gene from the bZIP transcription factor family was obtained by screening the transcriptome database of the wheat (Triticum aestivum) infected by leaf rust fungus (Puccinia triticina). The expression patterns were visualized using heatmap, and confirmed by qRT-PCR. The expression of TabZIP3 was up-regulated after 8, 12 and 16 h post inoculation in the incompatible combination, and was obviously higher than that of the compatible combination (P<0.05). TabZIP3 was predicted to contain salicylic acid (SA) response elements, and the expression could be induced by spraying exogenous SA on the leaf surface. By using Barley stripe mosaic virus (BSMV) as a vector, the virus-induced gene silencing (VIGS) technique was used to silence TabZIP3, followed by Puccinia triticina inoculation. Hypersensitive response (HR) area and the number of haustorial mother cell (HMC) of single infection sites was much higher than that of control at 48 and 96 h (P<0.05). The research results showed that the SA inducible TabZIP3 might participated in the induction of basic defense response against Puccinia triticina infection in wheat, and might played a positive regulation role in HR response induced by Puccinia triticina. The results lay a foundational data for further study on the mechanism of the transcription factor TabZIP3 in wheat resistance to Puccinia triticina infection, and also provide an excellent candidate gene for breeding new wheat varieties resistant to Puccinia triticina.
刘娜, 孙天杰, 陈琰, 侯春燕, 韩胜芳, 王冬梅. 小麦与叶锈菌互作过程中TabZIP3的功能研究[J]. 农业生物技术学报, 2020, 28(2): 201-210.
LIU Na, SUN Tian-Jie, CHEN Yan, HOU Chun-Yan, HAN Sheng-Fang, WANG Dong-Mei. Study on the Function of TabZIP3 in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina. 农业生物技术学报, 2020, 28(2): 201-210.
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