Polymorphism of miR-486 and Its Expression Pattern in Bashby Sheep (Ovis aries)
ZHANG Wei1,2, WANG Shi-Yin1,*, SHI Guo-Qing2,*, DENG Shuang-Yi1, LIU Xiao-Na1, YANG Li-Wei1
1 Xinjiang Agricultural Professional Technological College, Changji 831100, China; 2 Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China
Abstract：miR-486 is expressed in animal muscle at a high level and plays a key role in growth and development of myoblast in different tissues. To illuminate the polymorphism and expression pattern of miR-486 in Bashby sheep (Ovis aries), the stem-loop qRT-PCR was used to detect the expression levels in different tissues, and in skeletal muscle tissues of 8 fetal and postnatal periods of Bashby sheep. Then the mutation of miR-486 sequence in Bashby sheep group and its effect on cutting process of precursor miR-486 (pre-miR-486) in skeletal muscle cell were also researched. The result showed that the miR-486 was expressed at a high level in myocardium and skeletal muscle of the 100 d of fetal period of Bashby sheep, moderately expressed in stomach and intestines, and lowly expressed in liver, spleen, lung, skin and brain tissues; The expression levels of miR-486 in skeletal muscle of the 40~100 d of fetal periods were increased gradually, to a peak level on the 100 d of fetal periods then decreased gradually, and to a minimum level on the 120 d of fetal periods then increased continually until the 90 d of postnatal period; A G>C mutation was detected in pre-miR-486 sequence, which located at the first base of upstream of miR-486 mature sequence, the GG, GC and CC genotype could be detected in Bashby sheep group, but 91% individual of them were GC genotype, the further investigation indicated that the CC genotype could significantly up regulate the level of miR-486 in skeletal muscle cell (P<0.05). The present research could provide basic data for further uncovering the regulation mechanism of miR-486 in development of Bashby sheep skeletal muscle and relationship between its expression and excellent meat quality traits of Bashby sheep.
张伟, 王世银, 石国庆, 邓双义, 刘晓娜, 杨力伟. 巴什拜羊miR-486多态性及其表达规律研究[J]. 农业生物技术学报, 2020, 28(1): 92-100.
ZHANG Wei, WANG Shi-Yin, SHI Guo-Qing, DENG Shuang-Yi, LIU Xiao-Na, YANG Li-Wei. Polymorphism of miR-486 and Its Expression Pattern in Bashby Sheep (Ovis aries). 农业生物技术学报, 2020, 28(1): 92-100.
[1] 巴吐尔·阿不力克木, 帕提姑·阿布都克热, 肉孜阿吉, 等. 2012. 巴什拜羊肉不同部位的品种特性分析[J]. 新疆农业科学, 49(9): 1734-1741. (Batuer·Abulikemu, Patigu Abudoukere, Rouziaji, et al.2012. Analysis of quality characteristics of lamb from different anatomical locations of Bashbay sheep[J]. Xinjiang Agricultural Sciences, 49(9): 1734-1741.) [2] 甘尚权, 张伟, 沈敏, 等. 2013. 绵羊X染色体59383635位点多态性与脂尾性状的相关性分析[J]. 遗传, 35(10): 1209-1216. (Gan S Q, Zhang W, Shen M, et al.2013. Correlation analysis between polymorphism of the 59383635th locus on X chromosome and fat-tail trait in sheep[J]. Hereditas (Beijing), 35(10): 1209-1216.) [3] 韩业东, 决肯·阿尼瓦什, 帕娜尔·依都拉, 等. 2011. 巴什拜羊群体遗传多样性与遗传分化的研究[J]. 南京农业大学学报, 34(1): 123-127. (Han Y D, Jueken·Aniwashi, Panaer·Yidula, et al.2010. Study on genetic diversity and genetic differentiation among Bashibai sheep populations[J]. Journal of Nanjing Agricultural University, 34(1): 123-127.) [4] 决肯·阿尼瓦什, 克木尼斯汗, 海拉提, 等. 2010a. 巴什拜羊瘦肉型新品系的培育及其生产性能分析[J]. 新疆农业科学, 47(2): 406-409. (Jueken·Aniwashi, Kemunisihan, Hailati, et al.2010. Analysis of breeding and production performance of new strain lean meat type Bashbay sheep[J]. Xinjiang Agricultural Sciences, 47(2): 406-409.) [5] 决肯·阿尼瓦什, 韦涛, 李齐发, 等. 2010b. 巴什拜羊群体双肌基因基因型检测[J]. 新疆农业科学, 47(9): 1902-1906. (Jueken·Aniwashi, Wei T, Li Q F, et al.2010. Analysis of breeding and production performance of new strain lean meat type Bashbay sheep[J]. Xinjiang Agricultural Sciences, 47(9): 1902-1906.) [6] 决肯·阿尼瓦什, 克木尼斯汗·加汗, 海拉提, 等. 2010c. 导入野生盘羊瘦肉基因培育巴什拜羊新品系[J]. 新疆农业大学学报, 33(5): 427-430. (Jueken·Aniwashi, Kemunisihan·Jiahan, Hailati, et al.2010. Rearing new strain of Bashiibai sheep by infusing lean-meat gene from wild Ovis ammon[J]. Journal of Xinjiang Agricultural University, 33(5): 427-430.) [7] 张伟, 王世银, 邓双义, 等. 2018. 巴什拜羊骨骼肌不同发育阶段差异表达miRNA研究[J]. 农业生物技术学报, 26(1): 104-112. (Zhang W, Wang S Y, Deng S Y, et al.2018. Research of differentially expressed miRNA of skeletal muscle during different development stages in Bashbay sheep (Ovis aries)[J]. Journal of Agricultural Biotechnology, 26(1): 104-112.) [8] Abmayr S M, Pavlath G K.2012. Myoblast fusion: Lessons from flies and mice[J]. Development, 139(4): 641-656. [9] Alexander M S, Casar J C, Motohashi N, et al.2011. Regulation of DMD pathology by an ankyrin-encoded miRNA[J]. Skeletal Muscle, 1(1): 27. [10] Bensen J T, Chiu K T, Nyante S J, et al.2013. Association of germline microRNA SNPs in pre-miRNA flanking region and breast cancer risk and survival: The Carolina breast cancer study[J]. Cancer Causes Control, 24(6): 1099-1109. [11] Chen J F, Mandel E M, Thomson J M, et al.2006. The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation[J]. Nature Genetics, 38(2): 228-233. [12] Clop A, Marcq F, Takeda H, et al.2006. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep[J]. Nature Genetics, 38(7): 813-818. [13] Dey B K, Gagan J, Dutta A.2011. miR-206 and -486 induce myoblast differentiation by downregulating Pax7[J]. Molecular and Cellular, 31(1): 203-214. [14] Dikeakos P, Theodoropoulos G, Rizos S, et al.2014. Association of the miR-146aC>G, miR-149T>C, and miR-196a2T>C polymorphisms with gastric cancer risk and survival in the Greek population[J]. Molecular Biology Reports, 41(2): 1075-1080. [15] Eric M S, Jason R O, Viviana M, et al.2010. Regulation of PI3-kinase/Akt signaling by muscle-enriched microRNA-486[J]. Proceedings of the National Academy of Sciences of the USA, 107(9): 4218-4223. [16] Hitachi K, Nakatani M, Tsuchida K.2014. Myostatin signaling regulates Akt activity via the regulation of miR-486 expression[J]. International Journal of Biochemistry & Cell Biology, 47(2): 93-103. [17] Hong J S, Noh S H, Lee J S, et al.2012. Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression[J]. Gene, 506(1): 211-216. [18] Huang T H, Zhu M J, Li X Y, et al.2008. Discovery of porcine microRNAs and profiling from skeletal muscle tissues during development[J]. PLOS ONE, 3(9): e3225. [19] Lee J S, Kim J M, Lim K S, et al.2012. Effects of polymorphisms in the porcine microRNA MIR206/MIR133B cluster on muscle fiber and meat quality traits[J]. Animal Genetics, 44(1): 101-106. [20] Lewis B P, Burge C B, Bartel D P.2005. Conserved seed pairing, often flanked by adenosines indicates that thousands of human genes are microRNA targets[J]. Cell, 120(1): 15-20. [21] Li H Y, Xi Q Y, Xiong Y Y, et al.2012. Identification and comparison of microRNAs from skeletal muscle and adipose tissues from two porcine breeds[J]. Animal Genetics, 43(6): 704-713. [22] Ryan B M, Robles A I, Harris C C.2010. Genetic variation in microRNA networks: The implications for cancer research[J]. Nature Reviews Cancer, 10(6): 389-402. [23] Small E M, O'Rourke J R, Moresi V, et al.2010. Regulation of PI3-kinase/Akt signaling by muscle-enriched microRNA-486[J]. Proceedings of the National Academy of Sciences of the USA, 107(9): 4218-4223. [24] Shukla G C, Singh J, Barik S.2011. MicroRNAs: Processing, maturation, target recognition and regulatory functions[J]. Molecular and Cellular Pharmacology, 3(3): 83-92. [25] Zhang W, Wang L M, Zhou P, et al.2015. Identification and analysis of genetic variations in pri-mirnas expressed specifically or at a high level in sheep skeletal muscle[J]. PLOS ONE, 10(2): e0117327.
