Tissue Expression and Polymorphism of PER1 Gene and Their Association with Seasonal Reproduction in Sheep (Ovis aries)
XIANG Guang-Ming1,2*, LIU Qiu-Yue2*, WANG Xiang-Yu2, DI Ran2, HU Wen-Ping2, MA Lin2, ZENG Xian-Yin1, CHU Ming-Xing2**, CAO Xiao-Han1**
1 Life Science College, Sichuan Agricultural University, Ya'an 625014, China; 2 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Abstract：In China, seasonal reproduction limits both diversification and intensification of sheep (Ovis aries) production, thus it is necessary to explore the key genes that affect the seasonal reproduction of sheep at the molecular genetic level. In order to explore the association between tissue expression levels and polymorphisms of period 1 gene (PER1) with seasonal reproduction in sheep, semi-quantitative reverse transcription and polymerase chain reaction (sqRT-PCR) and quantitative real-time PCR (qPCR) were performed to investigate the expression of PER1 gene in different tissues including pineal gland, hypothalamus, pituitary, ovary, oviduct and uterus in Small Tail Han sheep and Sunite sheep respectively. Seasonal estrous breeds (Prairie Tibetan sheep, Sunite sheep and Tan sheep) and year-round estrous breeds (Small Tail Han sheep, Hu and Celeblack sheep) were selected, and Sequenom MassARRAY®SNP assay was applied to genotype 2 single nucleotide polymorphism sites (SNPs) of PER1 gene. Gene and genotype frequencies of those 2 sites were analyzed in sheep with different breeding traits. The results showed that PER1 expressed in all tissues selected. Expression levels of PER1 in Sunite sheep was higher than that of Small Tail Han sheep in all tested tissues, and there were significant differences in pineal gland, ovary, and uterus (P<0.05). There were three genotypes in both year-round estrous breeds and seasonal estrous breeds. The allele frequencies of the PER1 g.27342699G>A was significantly different between year-round estrous and seasonal estrous sheep (P<0.05). Association analysis indicated that there were no significant correlation between the polymorphisms of the 2 SNPs in PER1 gene and the litter size of each parity in Small Tail Han sheep. In conclusion, the expression of PER1 in Sunite sheep was higher than that of Small Tail Han sheep in the tissues relating with seasonal reproduction, which showed significant differences in pineal, ovary and uterus. Therefore, the higher level of PER1 gene was probably related to the seasonal estrus regulation, which will provide a theoretical basis for studying the seasonal reproduction mechanism of sheep.
向光明, 刘秋月, 王翔宇, 狄冉, 胡文萍, 马琳, 曾宪垠, 储明星, 曹晓涵. PER1基因组织表达及多态性与绵羊季节性繁殖的相关性研究[J]. 农业生物技术学报, 2019, 27(7): 1215-1223.
XIANG Guang-Ming, LIU Qiu-Yue, WANG Xiang-Yu, DI Ran, HU Wen-Ping, MA Lin, ZENG Xian-Yin, CHU Ming-Xing, CAO Xiao-Han. Tissue Expression and Polymorphism of PER1 Gene and Their Association with Seasonal Reproduction in Sheep (Ovis aries). 农业生物技术学报, 2019, 27(7): 1215-1223.
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