Effect of Genetic Variation of SFRP5 gene on Breast Muscle Quality of Changshun Blue-eggshell Chicken (Gallus gallus domesticus)
QIN Yuan-Yu, ZHANG Yi-Yu*, LUO Hua-Lun, WU Lei
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract：Secreted frizzled-related protein 5 (SFRP5) is a crucial anti-inflammatory adipokine secreted from white adipose tissue. It can regulate lipid metabolism, reduce insulin resistance, and keep inflammation balance by Wnt singal pathway. To investigate the relationship between SFRP5 gene polymorphism and muscle quality in Changshun Blue-eggshell chicken (Gallus gallus domesticus), and find molecular markers related to muscle quality. In this study, the single nucleotide polymorphisms (SNPs) of SFRP5 gene were detected by direct sequencing, and its correlation with the meat quality of chest muscle in Changshun Blue-eggshell chicken was analyzed. The results showed that 2 SNPs (g.23253254G>A and g.23253269T>G) were firstly found in SFRP5 gene exon 3. The dominant genotype and dominant allele of the g.23253254G>A locus was GG genotype and G allele, and its frequencies were 0.569 and 0.756, respectively. The g.23253269T>G locus was TT genotype and T allele with frequencies were 0.681 and 0.812, respectively. Two mutation sites of SFRP5 gene were synonymous mutations. Polymorphic information content (PIC) of the 2 loci were between 0.25 and 0.5, and belong to moderate polymorphic loci. The chi-square test indicated that the genotypes distribution of 2 SNPs (g.23253254G>A and g.23253269T>G) were agree with the Hardy-Weinberg equilibrium (P>0.05). There was no strong linkage disequilibrium (LD) between each SNP. Among the flock of Changshun Blue-eggshell chicken, 3 haplotypes and 6 diplotypes were formed on SFRP5 gene. The frequency of 3 haplotypes H1, H2 and H3 were 0.569, 0.187 and 0.244 respectively, and then the frequency of 6 diplotypes H1H1, H1H2, H1H3, H2H2, H2H3 and H3H3 were 0.313, 0.200, 0.313, 0.056, 0.063 and 0.056, respectively. Genetic correlation analysis demonstated that g.23253254 G>A locus was significantly correlated with crude fat of chest muscle (P<0.05), and further the diplotype H3H3 was obviously higher crude fat content than other 5 diplotypes (P<0.05). The present research revealed that SFRP5 gene had significant genotype effects on chicken meat quality and also suggested that both g.23253254 G>A and g.23253269 T>G variant sites of SFRP5 gene may be used as molecular markers to improve meat quality in Changshun Blue-eggshell chicken. This study provide theoretical basis for further exploring the molecular mechanism of SFRP5 gene variation in poultry muscle quality.
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