Abstract：Abstract Peroxisome proliferator-activated receptor α(PPARα) gene exon5 polymorphism was detected by PCR-SSCP and DNA sequencing in total 717 individuals, six breeds(Bos taurus) of Qinchuan cattle, Nanyang cattle, Jiaxian Red cattle, Luxi cattle, Xianan cattle and Angus, and its correlation of this SNP with some carcass and meat traits of 108 Qinchuan cattle was analyzed. T→C mutation was detected at 138 bp in PPARα gene exon5. Chi-square test showed that locus was not in Hardy-Weinberg equilibrium(P＜0.05) in Qinchuan cattle and Xianan cattle, but was in Hardy-Weinberg equilibrium(P＞0.05) in other breeds. In the six breeds, the polymorphism information contents(PIC) were moderate polymorphisms(0.25＜PIC＜0.50). Correlation analysis showed that AA genetype was significantly affected by back fat thickness and eye muscle area(P＜0.05); and BB genetype was significantly affected by water holding capacity(WHC )(P<0.05), showing this locus may be a major QTN or tightly linked back fat thickness, eye muscle area and WHC, it can be a candidate molecular marker for the beef cattle breeding.
