Functional Marker Mapping and Expression Characteristics Analysis of Gene TaDREB1 in Wheat (Triticum aestivum)
LEI Meng-Lin1,2, MAO Xin-Guo2, CHANG Xiao-Ping2, LIU Xia1, JING Rui-Lian2*
1 Key Laboratory of Crop Genetics and Molecular Improvement of Shanxi Province / Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture/Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：DREB (dehydration responsive element binding) in plant plays important roles in resisting drought, high salinity and low temperature, etc. However, the further study on its family showed that the resistance of this gene to different crops was complicated and limited. TaDREB1 was cloned by hexaploid wheat (Triticum aestivum) and its diploid and tetraploid wild relative species as materials (GenBank No. DQ195070.1). It was found that there were 3 DNA sequences in the hexaploid wheat, corresponding to the 3 genomes A, B and D, and were named as TaDREB1-a/b/d, respectively. Develop functional markers according to polymorphic sites of TaDREB1-b between different materials, TaDREB1-b was mapped using a doubled haploid (DH) and recombinant inbred lines (RIL) population. TaDREB1-b was mapped in the intervals between markers P2449-185 and WMC231 on chromosome 3B, with 19.2 and 16.0 cM from the flanking markers using DH population, and mapped in the intervals between markers Xfbb117 and Xgwm566 on chromosome 3B, with 3.4 and 6.4 cM from the flanking markers using RIL population; At the same time, the specific primers of TaDREB1-a/b/d were designed by using wheat variety Hanxuan 10 as the material, and the expression pattern of TaDREB1-a/b/d under 4 stresses was analyzed by qRT-PCR. The expression peak and degree of TaDREB1-a/b/d were different in leaves and roots. TaDREB1-a/b expression were up-regulated in leaves under PEG, NaCl and ABA condition, while the expression of TaDREB1-d were down-regulated. TaDREB1-a/b/d was up-regulated under low temperature in leaves. In roots, the expression of TaDREB1-a/b/d were up-regulated in all condition. TaDREB1-a/b/d were all involved in responding to PEG, NaCl, ABA and 4 ℃ stresses, but different in expression patterns. The functional markers developed in this study provides the tools for molecular marker-assisted selective breeding. Meanwhile, the relationship between TaDREB1-a/b/d and abiotic stress response is preliminarily explained at the genome level, laying a foundation for in-depth exploration of the molecular mechanism of wheat stress resistance.
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