Abstract：SnRK2 (sucrose non-fermenting-1-related protein kinase) is a kind of plant-specific Ser/Thr protein kinase, which plays an important role in plant stress resistance. In order to explore the number, structure and expression difference of SnRK2 gene family of strawberry (Fragaria vesca) in response to abiotic stress, based on the conserved sequences of SnRK2 genes in Arabidopsis thaliala and rice (Oryza sativa).Using bioinformatic tools, the homologous comparison, gene structure, systematic evolution, conserved motif, physical and chemical properties of protein, protein secondary structure and sequential action elements of strawberry SnRK2 gene family were analyzed, and qRT-PCR was used to analyze the gene expression under simulated stress. Ten members of SnRK2 gene family were identified from strawberry genome database, divided into 3 subfamilies, the number of coding amino acids is 258~366, and the molecular weight was 29 469.75~41 481.68 D, the theoretical isoelectric points were between 4.68 and 9.10, which were distributed on 4 chromosomes of strawberry. Gene structure and motif analysis showed that most genes had 9 exons, and the distribution of motif in the same subfamily was basically similar. The results of subcellular localization prediction showed that FvSnRK2 gene family was mainly expressed in cytoplasm. The secondary structures of protein were mainly α-helix and irregular curl. Analysis of the upstream 2 kb cis-acting element found that all members of the FvSnRK2 gene family contain MYB and MYC transcription factor response elements. The results of qRT-PCR analysis showed that the relative expression of 5 genes in FvSnRK2 gene family reached the peaks at 12 h after 10 % PEG6000 processing, and the response of FvSnRK2.4 was the most obvious, which was 15 times higher than that at 0 h. After 100 μmol/L ABA (abscisic acid) treatment, the relative expression of 6 genes was the highest at 24 h, and 2 genes had the highest relative expression at 12 h, FvSnRK2.5 and FvSnRK2.9 responsed strongly, which were 16.2 times and 42.4 times higher than that at 0 h, respectively. The relative expression of 7 genes was highest at 2 h after 200 mmol/L NaCl treatment, the response of FvSnRK2.10 was 65.2 times higher than that at 0 h, and FvSnRK2.5 was the highest at 12 h, which was 94.7 times higher than that at 0 h. FvSnRK2.5 and FvSnRK2.10 had strong response after ABA and NaCl treatment, and played important roles in stress resistance signal regulation in strawberry. The present research provides theoretical reference for functional verification and breeding of strawberry SnRK2 gene.
刘涛, 王萍萍, 何红红, 梁国平, 高雪琴, 卢世雄, 陈佰鸿, 毛娟. 草莓SnRK2基因家族的鉴定与表达分析[J]. 农业生物技术学报, 2019, 27(12): 2150-2163.
LIU Tao, WANG Ping-Ping, HE Hong-Hong, LIANG Guo-Ping, GAO Xue-Qin, LU Shi-Xiong, CHEN Bai-Hong, MAO Juan. Identification and Expression Analysis of SnRK2 Gene Family in Strawberry (Fragaria vesca). 农业生物技术学报, 2019, 27(12): 2150-2163.
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