联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月18日 星期一
  2018, Vol. 26 Issue (8): 1410-1418    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
樱桃谷鸭源鹅出血性多瘤病毒(GHPV)全基因组克隆与分析
万春和1,陈翠腾2,刘荣昌2,程龙飞1,施少华1,傅光华1,陈红梅1,傅秋玲2,黄瑜1
1. 福建省农业科学院畜牧兽医研究所
2. 福建省农业科学院 畜牧兽医研究所
Cloning and Analysis of Whole Genome Sequences in Cherry Valley Duck (Anas platyrhynchos domestica) Origin Goose hemorrhagic polyomavirus (GHPV)
全文: PDF (3135 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 摘 要 鹅出血性多瘤病毒(Goose hemorrhagic polyomavirus, GHPV)为近年来我国鸭群新发病原。为明确我国鸭源GHPV基因组特征,本研究利用分段PCR扩增技术克隆获得樱桃谷鸭(Anas platyrhynchos domestica)源GHPV(FJ201601株)全基因组序列,并对其进行核苷酸同源性比较和遗传进化分析。结果表明,克隆的GHPV(FJ201601株)全基因组序列长为5 254 bp(GenBank登录号MG544856),(G+C)%含量为48.01%,编码的6个主要功能基因(ORF-X, VP2, VP3, VP1, Large T和Small T)长度分别为510、981、654、1 062、1 911和483 bp。核苷酸同源性分析表明,FJ201601株和其他不同地区、不同宿主来源GHPV相互之间基因组核苷酸同源性较高(99.7%以上);其6个主要功能基因和其他不同地区、不同宿主来源GHPV相应基因的核苷酸(99.4%以上)和氨基酸同源性(98.2%以上)均极高。从全基因组及其编码的6个主要功能基因遗传进化关系可见,GHPV各分离株在基因组上亲缘关系较近,6个主要功能基因在遗传进化上差异不大,但北京鸭源GHPV和樱桃谷鸭源GHPV在遗传进化上仍存在细微差别,且不同宿主(多品种鸭和鹅)源GHPV在遗传进化上不存在宿主特异性。本研究丰富了GHPV分子流行病学数据,明确了GHPV基因组分子特征,为后续开展GHPV相关研究提供参考。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
万春和
陈翠腾
刘荣昌
程龙飞
施少华
傅光华
陈红梅
傅秋玲
黄瑜
关键词 鹅出血性多瘤病毒(GHPV)樱桃谷鸭源基因组序列分析遗传进化    
Abstract:Abstract Goose hemorrhagic polyomavirus (GHPV) is a newly identified emerging infectious pathogen in ducks in recent years, China. In order to understand the genomic characterization of Cherry Valley duck (Anas platyrhynchos domestica) origin GHPV (designated as strain FJ201601), the whole genome of GHPV strain FJ201601 was amplified by PCR with five fragments by using specific primers. The obtained sequences were cloned and verified by Sanger sequence, and then the positive results were assembled. The results showed that the genome length of strain FJ201601 comprised of 5 254 nucleotides (GenBank accession number MG544856), with(G+C)% content at 48.01%, coding for 6 function genes with ORF-X (510 bp, 169 aa), VP2(981 bp, 326 aa), VP3(654 bp, 217 aa), VP1(1062 bp, 353 aa), Large T(1911 bp, 636 aa) and Small T(483 bp, 160 aa), respectively. Genomic nucleotide comparison demonstrated that GHPV strain FJ201601 shared nucleotide identity higher than 99.7% with other GHPV isolates, which were collected from different hosts and regions. The GHPV six functional genes were all showed high nucleotide and amino acids identity at ORF-X (99.4%~99.8% and 98.2%~99.4%), VP2 (99.7%~99.9% and 99.7%~100%), VP3 (99.7%~99.8% and 99.5%~100%), VP1 (99.5%~99.8% and 99.7%~100%), Large T (99.7%~100% and 99.8%~100%) and Small T (99.6%~100% and 99.4%~100%), respectively. Phylogenetic analysis indicated that GHPV isolates shared very closer evolution distance. However, there were subtle differences in genetic evolution different did not consistent with different hosts (different duck and goose species) and regions, the same as the Chinese Pekin ducks-origin GHPV and Cherry Valley ducks-origin GHPV. This study lays a good foundation for further epidemiological investigation and pathogenic mechanism study of the Goose hemorrhagic polyomavirus.
Key wordsGoose hemorrhagic polyomavirus (GHPV)    Cherry Valley duck origin    Genome    Sequence analysis    Phylogenetic analysis
收稿日期: 2017-11-28      出版日期: 2018-07-20
基金资助:国家自然科学基金;国家水禽产业体系;福建省农业科学院青年科技创新团队;福建省农业科学院青年科技英才项目;福建省属公益类项目
通讯作者: 黄瑜     E-mail: huangyu_815@163.com
引用本文:   
万春和 陈翠腾 刘荣昌 程龙飞 施少华 傅光华 陈红梅 傅秋玲 黄瑜. 樱桃谷鸭源鹅出血性多瘤病毒(GHPV)全基因组克隆与分析[J]. , 2018, 26(8): 1410-1418.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I8/1410
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn