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2025年8月30日 星期六
  2018, Vol. 26 Issue (6): 987-994    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
阿特拉津降解菌LY-2的分离鉴定及其对污染土壤的修复
李阳阳,张金波,沙君雪,史琛琛,迟宝岩,高继国,李海涛,刘荣梅
东北农业大学
Isolation and Identification of Atrazine-Degrading Bacterial Strain LY-2 and its Bioremediation to Contaminated Soil
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摘要 摘 要 除草剂阿特拉津(2-氯-4-乙胺基-6-异丙胺基-1,3,5-三嗪, 2-chloro-4-ethylamino-6-isopropyl-amino-1,3,5-triazine, Atrazine)在很多国家普遍使用,对自然环境和人类健康造成威胁。本研究利用富集法,从黑龙江省哈尔滨市巴彦县施用多年阿特拉津的玉米地表层土中,分离出高效降解阿特拉津的菌株LY-2。LY-2以阿特拉津为唯一氮源,初步鉴定属于肠杆菌属(Enterobacter sp.)。PCR检测结果表明,LY-2含有阿特拉津降解相关的基因—阿特拉津氯水解酶基因(atrazine chlorohydrolase gene, atzA)、羟基阿特拉津脱乙胺基水解酶基因(hydroxyatrazine N-ethylaminohydrolase gene, atzB)和N-异丙基氰尿酰氨异丙氨基水解酶基因(N-isopropylammelide isopropylaminohydrolase gene, atzC)。LY-2在48 h内对100 mg/L阿特拉津降解率为98.7%;适宜温度为25~35 ℃,适宜酸碱度为pH 6~9;外加氮源没有降低该菌株对阿特拉津的降解率。土壤修复实验结果显示,培养7 d后,LY-2菌株对阿特拉津污染土壤(100 mg/kg)的降解率高达86.7%,培养14 d后,降解率高达90.1%。LY-2菌株在相对较短的时间内表现出良好的修复效果,在修复阿特拉津污染的土壤方面具有潜在的应用价值。
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李阳阳
张金波
沙君雪
史琛琛
迟宝岩
高继国
李海涛
刘荣梅
关键词 阿特拉津肠杆菌降解生物修复    
Abstract:Abstract Atrazine (2-chloro-4-ethylamino-6-isopropyl-amino-1,3,5-triazine) is widely used in many countries, and has great threat to the environment and human beings. In order to obtain a highly efficient atrazine-degrading bacteria, the present study isolated a bacterial strain (LY-2) which could use atrazine as a sole nitrogen source to grow by enrichment culture. The source was from the surface soil of maize field where atrazine had been used for many years. LY-2 was identified as Enterobacter sp. strain. It was the first reported Enterobacter sp. strain in detail that could degrade atrazine. The results of PCR test showed that LY-2 contained atrazine-degrading gene atzA, atzB, and atzC, which were involved in the degradation of atrazine to cyanuric acid. The strain LY-2 could degrade 98.7% atrazine (100 mg/L) within 48 h. The optimum temperature range of the strain was 25~35 ℃ and the optimum pH range was 6~9. Additional nitrogen sources did not affect the degradation of atrazine. The experiment of soil remediation showed that the highest degradation rate of the strain was 86.7% after incubation for 7 d, and the highest degradation rate of strain was 90.1% after incubation for 14 d, the concentration of atrazine in contaminated soil was 100 mg/kg. The strain LY-2 shows good bioremediation effect in a relatively short period of time and has a better potential for bioremediation of atrazine-contaminated soil.
Key wordsAtrazine    Enterobacter sp.    Degradation    Bioremediation
收稿日期: 2017-11-17      出版日期: 2018-05-21
ZTFLH:  Q89  
基金资助:黑龙江省教育厅基金
通讯作者: 刘荣梅     E-mail: liurongmei@neau.edu.cn
引用本文:   
李阳阳 张金波 沙君雪 史琛琛 迟宝岩 高继国 李海涛 刘荣梅. 阿特拉津降解菌LY-2的分离鉴定及其对污染土壤的修复[J]. , 2018, 26(6): 987-994.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I6/987
 
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