Abstract：Sucrose is one of the main assimilates in plant photosynthesis process. The effective use of sucrose in plant reproduction plays important roles in the process of fertilization and fruit-setting. Invertase (INV), as a key enzyme of sucrose metabolism, regulate the hydrolysis of sucrose into glucose and fructose. According to the cellular localization, INVs are further divided as cytoplasmic invertase (CIN), vacuolar invertase (VIN), and cell wall invertase (CWIN). In order to understand the physiological and molecular mechanism of sucrose metabolism involved in the process of male sterility of pepper (Capsicum annuum) induced by pollen abortion, the INV enzyme activities, INV genes expression pattern and their correlation with pepper male sterility were analyzed. A pair of pepper male sterile line 131A and maintainer line 131B (fertile) were used as investigating materials in this study. The parameters including INV enzyme activities, VIN and CWIN gene identification in pepper genome, and CWIN encoding gene expression pattern by qRT-PCR, were comparatively analyzed in different tissues and developmental stages of pepper flower. The results showed that the CWIN activities in the big bud and flower of the maintainer line 131B were significantly higher than those in the male sterile line 131A (P＜0.001 and P＜0.01, respectively), which were closely correlated with the high expression level of pepper CaCWIN encoding genes CaCWIN3 and CaCWIN4. According to the results, CaCWIN3 and CaCWIN4 were likely the key functional genes involved in the process of male sterility of pepper. These results shed light on the further investigation of molecular regulation mechanism of capsicum male sterility.
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