Abstract：Abstract Stearyl-CoA desaturase (SCD) is a key enzyme in the biosynthesis ofmonounsaturated fatty acids in organisms. It has a wide range of physiological functions and plays an important role in the regulation of lipid dynamic equilibrium, fat generation and lipid oxidation. Transcriptomic analysis of livers of pre-laying (20 weeks old) and peak-laying (30 weeks old) hens showed that the expression level of SCD gene was significantly higher in the liver of laying hens than that in pre-laying hens. To further understand the regulation mechanism of expression and biological functions of the gene in chicken, real-time quantitative PCR (qPCR) was used to analyze the expression patterns of SCD in different tissues and various developmental stages. The regulation mechanism of SCD was exploried by a series of experiments in vivo and in vitro. The results showed that the SCD was extensively expressed in various tissues with relatively higher expression levels in liver, abdominal fat and kidney, and also relatively higher expression levels in peak-laying hens than that in young poults. The expression of the SCD gene was significantly regulated by estrogen. This study indicated that expression of the SCD gene is regulated by estrogen and might involve in liver lipid metabolism in chicken.
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