Abstract：Animal embryonic development is regulated by many genes. In order to explore the molecular mechanism of duck early embryonic development, we constructed the subtractive library of duck early embryonic development related genes to screen the related candidate genes of duck early embryonic development. Two different periods (6 and 20 h) duck (Anas platyrhynchos) embryo were used respectively as the driver and tester, and a designated duplex-specific nuclease(DSN) mediated subtractive hybridization method was employed to construct the subtractive library of duck early embryonic development related genes, and the genes were sequenced and analyzed by bioinformatics. Results showed that 54 genes were obtained by DNA sequencing from 216 positive clones picked randomly. Their average length was 1 084 bp. Nucleotide BLAST homological analysis showed that 44 genes had similarities to known genes. Gene ontology showed that these genes were related with a variety of biological pathways，such as metabolism, establishment of cellular localization, biological regulation，etc. And these genes played very important roles during early embryonic development of duck in many different aspects (such as nervous system development, immune regulation, transcriptional regulation, etc.). We conclude that isolation and identification of these differentially expressed genes will contribute to the future related research of poultry early embryonic development.
