Establishmeut of Dynamic Models for Keratinase of Bacillus subtilis KD-N2 by Batch Fermentation using Feather andHuman Hair as Carbon and Nitrogen Substrate
Abstract：Keratinase batch cultivation in a 5 L fermentor was studied using Bacillus subtilis KD-N2 as fermentation bacteria, and feather and human hair as carbon and nitrogen substrates. The kinetic models were proposed for cell growth and product formation based on the Logistic and Luedeking-Piret equations. Parameters of the models were established with the experimental data. The models for cell growth and keratinase formation in feather substrate were described by equations of dX/dt =0.1013(1－X/ 0.3742)X and dP/dt =276.69dX/dt－3.6X ,respectively; for human hair substrate, the equations were of dX/dt =0.0728(1－X/ 0.197)X and dP/dt =422.34dX/dt + 5.187X.
