Institute for Horticultural Plants, China Agricultural University/ Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, Beijing 100193, China
Abstract： The regulative sequence(1 738 bp) of ferric chelate reductase (MxFRO2) gene from Malus xiaojinensis was cloned by genomic walking. In silico analysis of sequence suggested that the sequence contained several typical cis-acting elements, including multiple light responsive elements (LREs), ABA responsive element (ABRE), auxin inducible element, Cu responsive element (CuRE), TATA-box and CAAT-box. The promoter sequences of 8 members of Arabidopsis FRO gene family gained from TAIR website were predicted as well. A 1 644 bp and a 259 bp promoter sequence upstream 5' of translation start site of MxFRO2 were cloned and designated as MxFul and MxD1, respectively. MxFul-GFP and MxFul-GFP were constructed and transformed into Arabidopsis mesophyll protoplasts mediated by PEG. These results showed that MxFul and MxD1 could both drive the transient expression of GFP protein in Arabidopsis mesophyll protoplasts.
