摘要利用松材线虫(Bursaphelenchus xylophilus)匀浆液免疫BALB/c小鼠，取其脾细胞与SP2/0骨髓瘤细胞融合获得一株能稳定传代并分泌抗松材线虫单克隆抗体的杂交瘤细胞，命名为1D3，经ELISA检测其特异性好、效价达到1∶5.12×105，抗体类型为IgG1亚类。Western blot 分析表明，1D3 单克隆抗体株与松材线虫匀浆液有明显反应。单克隆抗体的制备成功为准确地鉴定松材线虫提供了一种快速、灵敏和有效的诊断方法。
Abstract：The pine wood nematode, Bursaphelenchus xylophilus, is one of the most important pathogens in pine trees. Exact, quick and sensitive method for identification and diagnosis of the nematode is the precondition for managing the nematode transmission. One hybrid cell lines, named as 1D3, secreting monoclonal antibody ( Mab ) to Bursaphelenchus xylophilus were developed by fusing splenocytes of BALB/c mice to mouse mycloma cell line SP2/0. The ELISA titer was 1∶5.12×105. This specific Mab was identified as IgG1.Western blotting analysis showed that the Mab could react with B. xylophilus. The production of monoclonal antibody to B.xylophilus provides a rapid and sensitive method for identification and diagnosis of the nematode.
