Abstract：Heat shock protein 90(HSP90) family is an evolutionarily conserved protein, to be involved in chaperone function, cell cycle control and antigen presentation. The cDNA sequences of heat shock protein 90 from Bursaphelenchus xylophilus, B. mucronatus and B. doui were cloned by RT-PCR and RACE methods, and named Bx-hsp90, Bm-hsp90, Bd-hsp90 (GenBank Accession No. GU013561, HMO34943 and HM347331) respectively. Theire full cDNA length were 2 255, 2 193 and 2 232 bp, the ORF were all 2 127 bp encoding 708 amino acid residues, 5'-untranslated region (UTR) was 33, 13 and 13 bp, and 3'-UTR was 95, 53 and 92 bp, respectively. And their full genomic length were 2 440, 2 388 and 2 407 bp, and all genomic sequences contained 3 introns. The amino acid sequence identities among Bx-hsp90, Bm-hsp90 and Bd-hsp90 was 98.78%, Evolutionary analyses showed that Bursaphelenchus xylophilus, B. mucronatus and B. doui had a close relationship with Caenorhabditis elegans, which might be indicative of their low evolution. Heat shock protein 90 from B. xylophilus, B. mucronatus and B. doui shared highly homologous, which made hsp90 to be a candidate gene for studying the biological evolution. The present study has a practical significance for understanding the invasive mechanism of B.xylophilus and lays a good foundation for understanding the environmental adaptabilities of these three plant parasitic nematodes.
代玲玲1,彭德良2,黄文坤3,彭焕4,龙海波2,王高峰2. 松材线虫及其近缘种热激蛋白90基因(hsp90)克隆与序列分析[J]. , 2011, 19(5): 916-923.
Lingling Dai1,De-liang Peng1, 1,Huan Peng 1, 1. Cloning and Sequence Analysis of Three New Heat Shock Protein 90 Genes(hsp90) from Bursaphelenchus xylophilus, B. mucronatus and B. doui. , 2011, 19(5): 916-923.
