Identification of HSF and HSP Gene Family in Pomacea canaliculata and Their Expression Pattern Under Temperature Stress
CAO Meng-Yu, FAN Yue-Yuan, YIN Chuan-Lin*, YU Xiao-Ping*
College of Life Sciences/Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, China
Abstract：As a serious global invasive species with strong adaptability to the environment, the golden apple snail (Pomacea canalicata) had spread to 16 provinces cities and regions in the southern China. Heat shock proteins (HSPs) are an important type of molecular chaperone proteins produced by organisms in response to environmental stress and environmental factor stimulation can induce its expression to cope with adverse effects of organism; the heat shock factor (HSF) is a key factor of transcription regulating of heat shock protein genes. In order to explore the role of HSF and HSP genes in the regulation of temperature tolerance of snails, in this study, only 1 HSF1 gene were identified by bioinformatics methods from the P. canaliculata as well as other 3 apple snails (P. maculata, Lanistes nyassanus, Marisa cornuarietis) which were in the same genus or family, and also 63, 66, 67, 64 HSP genes were identified, respectively. The amino acid length and gene structure of HSF1 gene in 4 snails were similar and highly conservative, which showed low gene diversity during the evolution of species. The HSP genes in the 4 snails had little difference, but the subfamily composition of the gene family was quite different. The HSP90 gene was relatively conserved, while HSP20 gene changed greatly. The HSP40 gene had the most members in the gene family among the 4 apple snails. Based on the gene expression of RNA-seq data, the results showed that the expression of HSF1 gene from high to low were gill, foot muscle, liver and ovary, respectively. Under high temperature stress, the HSF1 gene expression in 4 tissues were highly expressed, and the expression pattern was consistent in foot muscle and liver tissue. According to the HSP gene expression heat map, 17 HSP genes were specifically highly expressed under high temperature stress, results of 12 genes were consistent with the qPCR. The present study provides a reference basis for exploring the mechanism of HSF and HSP gene family in temperature tolerance.
曹梦宇, 范月圆, 尹传林, 俞晓平. 小管福寿螺HSF和HSP基因家族鉴定及其在温度胁迫下的表达模式[J]. 农业生物技术学报, 2023, 31(2): 370-382.
CAO Meng-Yu, FAN Yue-Yuan, YIN Chuan-Lin, YU Xiao-Ping. Identification of HSF and HSP Gene Family in Pomacea canaliculata and Their Expression Pattern Under Temperature Stress. 农业生物技术学报, 2023, 31(2): 370-382.
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